PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

RESUMO.- Assis Neto A.C., Morceli J.A.B., Fonseca R., Ambrósio C.E., Pereira F.T.V. & Miglino M.A. 2009. [Biometrics evolution of the embryonic and fetal annexes in cows obtained by natural mating, at 10 to 70 days of gestation.] Evolução morfométrica dos anexos embrionários e fetais bovinos obtidos por monta natural, com 10 a 70 dias da gestação. Pesquisa Veterinária Brasileira 29(10):859-862. Faculdade de Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho, Unidade Experimental de Dracena, Rodovia Comandante João Ribeiro de Barros Km 651, Dracena, SP 17900-000, Brazil. E-mail: antonioassis@dracena.unesp.br O período inicial da gestação de bovinos é caracterizado por grandes perdas embrionárias. Considerando a importância deste fator no âmbito da reprodução animal foram estudados os anexos embrionários e fetais bovinos fecundados por monta natural de 15-70 dias de gestação, com o objetivo de estabelecer parâmetros morfométricos da placenta na fase inicial da gestação. Com uso de um paquímetro foram realizadas mensurações do comprimento (crânio caudal), largura (latero lateral) e altura (dorso ventral) das membranas corioalantóide e amniótica. O início da formação dos cotilédones foi observado e quantificado, assim como, o peso placentário. O peso médio do saco gestacional aumentou com o evoluir da idade gestacional, entretanto, o crescimento foi acelerado a partir de 20-30 dias de gestação. O comprimento crânio caudal e dorso ventral da membrana corioalantóide e do âmnio apresentaram crescimento lento e gradual com o evoluir dos períodos gestacionais analisados. Com 30-40 dias de gestação, os primeiro cotilédones já eram visualizados e contatos com facilidade na superfície coriônica. Os períodos de crescimento coincidiram com os maiores índices de perdas gestacionais em bovinos. Os parâmetros aqui analisados poderão servir para futuras investigações dos anexos embrionários de organismos manipulados em laboratório.

• Placenta bovine extraembryonics membranes amnion chorion

Abstract in Portuguese:

ABSTRACT.- Assis Neto A.C., Morceli J.A.B., Fonseca R., Ambrósio C.E., Pereira F.T.V. & Miglino M.A. 2009. [Biometrics evolution of the embryonic and fetal annexes in cows obtained by natural mating, at 10 to 70 days of gestation.] Evolução morfométrica dos anexos embrionários e fetais bovinos obtidos por monta natural, com 10 a 70 dias da gestação. Pesquisa Veterinária Brasileira 29(10):859-862. Faculdade de Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho, Unidade Experimental de Dracena, Rodovia Comandante João Ribeiro de Barros Km 651, Dracena, SP 17900-000, Brazil. E-mail: antonioassis@dracena.unesp.br The main goal of this morphometrical study was to characterize the development of the extra-embryonic membranes of 15 to 70-day-old bovine embryos obtained by natural mating. With a millimeter paquimeter the cranio-caudal, latero-lateral and dorso-ventral measurements of chorion and amnion were determined. The development of the cotyledons and weight of the gestational sac were observed and quantified. The weight of the gestational sac increased during gestation; however, the growth was faster then 20 to 30 days. The cranial-caudal and dorsal-ventral length of the choriallantois and the amnion developed slowly and gradually with progress of the gestational period, and the cranio-caudal length decreased after 50 to 60 days of gestation, increasing in the next period. After 30 to 40 days of gestation, the first cotyledons were visualized and counted easily in the chorionic surface. The growth periods were the same of the main gestational losses in cattle. The parameters analyzed could be useful for investigations of extra-embryonic membranes in organisms manipulated in the laboratory.

Abstract in English:

ABSTRACT.- Ferreira S.R., Murakami A.E., Siqueira T.G.V., Santos J.M.G., Potença A. & Santos T.C. 2009. [Cellular immune response and hematological parameters of broilers from different age broiler breeders, fed with a sorghum meal with different yeast wall levels.] Níveis crescentes de parede de levedura sobre a resposta imune celular e perfil hematológico de frangos de corte. Pesquisa Veterinária Brasileira 29(9):725-730, Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, PR 87020-900, Brazil. E-mail: aemurakami@uem.br An experiment was carried out to evaluate the effect increasing levels of yeast wall and broiler breeders’ age levels on lymphoid organs weight, cellular immune response and hematological parameters in broilers. A total of 3,360 Cobb broilers were allotted, in a completely randomized design and a 2x5 factorial arrangement, and two controls, compound of two broiler breeders age (34 and 57 weeks of age) and five yeast wall levels (zero, one, two, three and four kg of yeast wall/ton of diet). Broiler breeders’ age affected all studied variables. The inclusion of 3kg of yeast wall/ton of meal increased, at 57 weeks age broiler breeders, more intense inflammatory reaction when compared to control diet; however the circulated heterophils and lymphocytes numbers were not increased. In conclusion, the use of yeast wall, associated or not to sorghum on broilers diet still needs complementary studies, for example, purified components of yeast wall (MOS and ß-glucano).

• Yeast wall levedura

Abstract in Portuguese:

RESUMO.- Ferreira S.R., Murakami A.E., Siqueira T.G.V., Santos J.M.G., Potença A. & Santos T.C. 2009. [Cellular immune response and hematological parameters of broilers from different age broiler breeders, fed with a sorghum meal with different yeast wall levels.] Níveis crescentes de parede de levedura sobre a resposta imune celular e perfil hematológico de frangos de corte. Pesquisa Veterinária Brasileira 29(9):725-730, Universidade Estadual de Maringá, Av. Colombo 5790, Maringá, PR 87020-900, Brazil. E-mail: aemurakami@uem.br Um experimento foi conduzido para avaliar o efeito de níveis crescentes de parede de levedura e idade das matrizes reprodutoras sobre o peso dos órgãos linfóides, a resposta imune celular e o perfil hematológico de frangos de corte. Foram utilizados 3.360 pintos de corte da linhagem Cobb, distribuídos em delineamento inteiramente casualisado, em esquema fatorial 2x5, mais dois controles, sendo duas idades de matrizes (34 e 57 semanas de idade) e cinco níveis de suplementação de parede de levedura (zero, um, dois, três e quatro kg de parede de levedura/tonelada de ração). A idade das matrizes influenciou a resposta de todas as variáveis. A inclusão de 3kg de parede de levedura/tonelada de ração promoveu, na progênie de reprodutoras de 57 semanas, reação inflamatória mais intensa quando comparada a dieta controle, no entanto não houve aumento significativo no número de heterófilos e linfócitos circulantes. Conclui-se que a utilização da parede de levedura associada ao sorgo ou não em rações de frangos de corte ainda necessita de estudos complementares, que incluam, por exemplo, os componentes purificados da parede de levedura (MOS e ß-glucano).

Abstract in English:

ABSTRACT.- Kondo K.R.J., Fonseca C.C., Da Matta S.L.P. & Viloria M.I.V. 2009. [Histomorphometric analysis of the extracellular matrix of popliteal lymph nodes from dogs naturally infected by Leishmania (L.) chagasi.] Análise histomorfométrica da matriz extracelular do linfonodo poplíteo de cães naturalmente infectados por Leishmania (L.) chagasi. Pesquisa Veterinária Brasileira 29(8):610-616. Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil. E-mail: krisregia@hotmail.com In the Americas, canine visceral leishmaniasis is caused by Leishmania (Leishmania) chagasi, an obligatory intracellular parasite of the phagocytic-monocytic system; the main histological changes associated with this disease occur in the lymphoid organs. Although dogs are considered to be the main carriers and disseminators of leishmaniasis in urban areas, there are few studies on the histopathologic and histomorphometric aspects in dogs naturally infected by L.chagasi analyzing the interaction between parasite and extracellular matrix. The current study characterize and quantify changes in the cellular and extracellular matrix (collagens type I and III) components of the popliteal lymph node from of 22 dogs with the natural infection by L. chagasi confirmed by indirect immuno-fluorescence assay (IFA) and compare theses findings with those fund in the popliteal lymph node from 10 non-infected dogs, that reacted negative in the IFA, and were clinically healthy. Lymph node fragments were longitudinally sliced and sections were processed for routine histopathology and stained by hematoxylin and eosin. For histomorphometry, additional sections from the same lymph node were fixed in glycol methacrylate and stained with toluidine blue. Lymph nodes from affected dogs were systemically enlarged, had increased numbers of lymphoid follicles, capsule hyperplasia and hypertrophy, and significant hyperplasia of lymphoid cells. In the lymph nodes from infected dogs, quantitative analyses of collagen fibers revealed predominance of type I collagen over type III fibers. These results demonstrate that dogs infected by L.chagasi experience degradation of the extracellular matrix components and consequently destruction of the lymphoid framework, thus altering nodal morphology.

• Leishmaniasis

Abstract in Portuguese:

RESUMO.- Kondo K.R.J., Fonseca C.C., Da Matta S.L.P. & Viloria M.I.V. 2009. [Histomorphometric analysis of the extracellular matrix of popliteal lymph nodes from dogs naturally infected by Leishmania (L.) chagasi.] Análise histomorfométrica da matriz extracelular do linfonodo poplíteo de cães naturalmente infectados por Leishmania (L.) chagasi. Pesquisa Veterinária Brasileira 29(8):610-616. Departamento de Veterinária, Universidade Federal de Viçosa, Av. Peter Henry Rolfs s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil. E-mail: krisregia@hotmail.com Nas Américas, a leishmaniose visceral canina é causada por Leishmania (Leishmania) chagasi, um protozoário intracelular obrigatório do sistema fagocítico mononuclear; as principais alterações histológicas associadas a essa doença ocorrem nos em órgãos linfóides. Apesar de o cão ser considerado o principal mantenedor e disseminador da leishmaniose no ambiente urbano, são escassos estudos dos aspectos histopatológicos e histomorfométricos, em cães naturalmente infectados com L. chagasi, que investiguem a interação entre o parasito e a matriz extracelular. Este estudo visou caracterizar e quantificar as alterações dos componentes celulares e da matriz extracelular (colágenos I e III) do linfonodo poplíteo de 22 cães com infecção natural por L. chagasi detectada através da reação de imunofluorescência indireta (RIF) e compará-las com as alterações encontradas no linfonodo poplíteo de 10 cães não-infectados, negativos na RIF e clinicamente saudáveis. Fragmentos dos linfonodos foram seccionados longitudinalmente, processados rotineiramente para exame histológico e corados por hematoxilina-eosina. Cortes adicionais do mesmo linfonodo incluídos em glicol metacrilato foram corados pelo azul de toluidina para histomorfometria. Linfonodos de cães infectados apresentaram linfadenopatia generalizada, aumento do tamanho e do número dos folículos linfóides, hipertrofia da cápsula e hiperplasia linfóide significativa. Nos linfonodos de cães do grupo infectado, a análise quantitativa de fibras colágenas mostrou significativo predomínio do colágeno I sobre o colágeno III. Esses resultados demonstram que cães infectados por L. chagasi apresentam degradação dos constituintes da matriz extracelular e conseqüente destruição do arcabouço linfóide, alterando a morfologia do órgão.

Abstract in English:

ABSTRACT.- Gabriel A.L., Kommers G.D., Masuda E.K, Fighera R.A., Piazer J.V.M., Barros C.S.L., Martins T.B. & Rosa F.B. 2009. [Clinical and hematological aspects and urinary bladder lesions in chronic spontaneous poisoning by Pteridium aquilinum in cattle.] Aspectos clínicos-hematológicos e lesões vesicais nas formas crônicas de intoxicação espontânea por Pteridium aquilinum em bovinos. Pesquisa Veterinária Brasileira 29(7):515-525. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: glaukommers@yahoo.com Spontaneous cases of chronic poisoning by Pteridium aquilinum in cattle were studied. The clinical forms of the disease were squamous cell carcinoma (SCCs) of the upper digestive tract (UDT) and bovine enzootic hematuria (BEH). The cases were from the midland Region of the Midwest of Rio Grande do Sul State, Brazil, and were submitted to the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria. Clinical signs and blood work were evaluated at terminal phase of disease. Cattle with UDT SCCs had progressive weigth loss, ruminal atony, cough, dysphagia, bloating, and regurgitation. In cattle with BEH, hematuria was observed in all cases, followed by progressive weight loss. Non-regenerative anemia was detected in 33.33% of the cattle with UDT SCCs form and in 66.66% of the cattle with BEH form. Changes in white blood count occurred in some cases but drop in lymphocyte numbers was uncommon in both forms of disease. For the morphological study, urinary bladders from 46 cattle with UDT SCCs and 11 cattle with BEH were analyzed. Grossly, 16/46 bladders from the UDT SCCs form had gross lesions (red or pale vesical nodules, hemorrhages, and papilomas; red urine was detected at necropsy of only three cases). In BEH form, the bladder had nodules, large neoplastic masses, red urine, papilomas, and hemorrhages. Pyelonephritis and hydronephrosis were seen in a few cases. Microscopically, in the UDT SCCs form, 44/46 (95.65%) bladders had 22 different types of morphological changes, characterized by neoplastic lesions (5/22) and non-neoplastic lesions (17/22); the latter were subdivided in non-neoplastic epithelial changes (6/17), general changes of the lamina propria (6/17), and inflammatory changes (5/17). The bladder changes in BEH form were of 19 different types, characterized by neoplastic lesions (5/19) and non-neoplastic lesions (14/19), which were subdivided in non-neoplastic epitelial changes (9/14), general changes of the lamina propria (3/14), and inflammatory changes (2/14). In BEH, mesenchymal neoplasms were more observed than epithelial ones, and most of them were malignant. Immunohistochemistry was utilized to characterize the histogenesis of poorly differentiated neoplasms. In conclusion, the morphological study demonstrated that urinary bladder lesions identical to the those seen in cattle with BEH, are very often present in cattle with the UDT SCCs form.

• Diseases of cattle poisonous plants; Pteridium aquilinum bovine enzootic hematuria squamous cell carcinomas of the digestive system veterinary pathology

Abstract in Portuguese:

ABSTRACT.- Gabriel A.L., Kommers G.D., Masuda E.K, Fighera R.A., Piazer J.V.M., Barros C.S.L., Martins T.B. & Rosa F.B. 2009. [Clinical and hematological aspects and urinary bladder lesions in chronic spontaneous poisoning by Pteridium aquilinum in cattle.] Aspectos clínicos-hematológicos e lesões vesicais nas formas crônicas de intoxicação espontânea por Pteridium aquilinum em bovinos. Pesquisa Veterinária Brasileira 29(7):515-525. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: glaukommers@yahoo.com Spontaneous cases of chronic poisoning by Pteridium aquilinum in cattle were studied. The clinical forms of the disease were squamous cell carcinoma (SCCs) of the upper digestive tract (UDT) and bovine enzootic hematuria (BEH). The cases were from the midland Region of the Midwest of Rio Grande do Sul State, Brazil, and were submitted to the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria. Clinical signs and blood work were evaluated at terminal phase of disease. Cattle with UDT SCCs had progressive weigth loss, ruminal atony, cough, dysphagia, bloating, and regurgitation. In cattle with BEH, hematuria was observed in all cases, followed by progressive weight loss. Non-regenerative anemia was detected in 33.33% of the cattle with UDT SCCs form and in 66.66% of the cattle with BEH form. Changes in white blood count occurred in some cases but drop in lymphocyte numbers was uncommon in both forms of disease. For the morphological study, urinary bladders from 46 cattle with UDT SCCs and 11 cattle with BEH were analyzed. Grossly, 16/46 bladders from the UDT SCCs form had gross lesions (red or pale vesical nodules, hemorrhages, and papilomas; red urine was detected at necropsy of only three cases). In BEH form, the bladder had nodules, large neoplastic masses, red urine, papilomas, and hemorrhages. Pyelonephritis and hydronephrosis were seen in a few cases. Microscopically, in the UDT SCCs form, 44/46 (95.65%) bladders had 22 different types of morphological changes, characterized by neoplastic lesions (5/22) and non-neoplastic lesions (17/22); the latter were subdivided in non-neoplastic epithelial changes (6/17), general changes of the lamina propria (6/17), and inflammatory changes (5/17). The bladder changes in BEH form were of 19 different types, characterized by neoplastic lesions (5/19) and non-neoplastic lesions (14/19), which were subdivided in non-neoplastic epitelial changes (9/14), general changes of the lamina propria (3/14), and inflammatory changes (2/14). In BEH, mesenchymal neoplasms were more observed than epithelial ones, and most of them were malignant. Immunohistochemistry was utilized to characterize the histogenesis of poorly differentiated neoplasms. In conclusion, the morphological study demonstrated that urinary bladder lesions identical to the those seen in cattle with BEH, are very often present in cattle with the UDT SCCs form.

Abstract in English:

ABSTRACT.- Godoy S.N., Alves V.A.F., Kanamura C.T. & Matushima E.R. 2009. [Main neoplasic processes in psittacines birds kept in captivity.] Principais processos neoplásicos encontrados em psitacídeos mantidos em cativeiro. Pesquisa Veterinária Brasileira 29(6):445-451. Instituto Brasileiro de Meio Ambiente e Recursos Naturais Renováveis, SHIN CA 02, Bloco D, Apto 408, Ed. Spazio Uno, Brasilia, DF 71.503-502, Brazil. E-mail: silng@uol.com.br An anatomopathologic study of 130 captivy psittacines from zoos, breeders and rehabilitation centers showed the presence of neoplastic disease in seven birds, totalizing 5.4% of the cases. The neoplasms were identified like cholangiocarcinoma, cholangioma, lymphoma, haemangiosarcoma and renal cell carcinoma. The cholangiocarcinoma was the tumor most commonly observed. it was present in birds. This study describes the macro and microscopic findings of these tumors; also reports diagnostic methods employed to identify these neoplasms.

• Neoplasms birds Psittacines pathology

Abstract in Portuguese:

ABSTRACT.- Godoy S.N., Alves V.A.F., Kanamura C.T. & Matushima E.R. 2009. [Main neoplasic processes in psittacines birds kept in captivity.] Principais processos neoplásicos encontrados em psitacídeos mantidos em cativeiro. Pesquisa Veterinária Brasileira 29(6):445-451. Instituto Brasileiro de Meio Ambiente e Recursos Naturais Renováveis, SHIN CA 02, Bloco D, Apto 408, Ed. Spazio Uno, Brasilia, DF 71.503-502, Brazil. E-mail: silng@uol.com.br An anatomopathologic study of 130 captivy psittacines from zoos, breeders and rehabilitation centers showed the presence of neoplastic disease in seven birds, totalizing 5.4% of the cases. The neoplasms were identified like cholangiocarcinoma, cholangioma, lymphoma, haemangiosarcoma and renal cell carcinoma. The cholangiocarcinoma was the tumor most commonly observed. it was present in birds. This study describes the macro and microscopic findings of these tumors; also reports diagnostic methods employed to identify these neoplasms.

Abstract in English:

ABSTRACT.- Figueiredo A.O., Pellegrin A.O., Gonçalves V.S.P., Freitas E.B., Monteiro L.A.R.C., Oliveira J.M. & Osório A.L.A.R. 2009. [Prevalence and risk factors for bovine leptospirosis in Mato Grosso do Sul, Brazil.] Prevalência e fatores de risco para a leptospirose em bovinos do Mato Grosso do Sul. Pesquisa Veterinária Brasileira 29(5):375-381. Programa Mestrado em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil. E-mail: line_figueiredo@yahoo.com.br The prevalence of anti-Leptospira spp. antibodies was estimated for female cattle aged 24 months or older. The sample comprised 178 herds from 22 counties in the state of Mato Grosso do Sul, Brazil. The risk factors associated with the presence of infeccion were investigated. A total of 2,573 blood serum samples were tested against 10 leptospira serovars using the microagglutination test (MAT). Titers of 100 or higher for one or more serovars were detected in 1,801 females (98.8%) from 161 herds (96.5%). Serovar Hardjo (65.6%) was the most frequent, followed by serovar Wolffi (12.3%). These results suggest that bovine leptospirosis is widespread in all the counties under study, with a high prevalence both at the animal and the herd level. Beef farms and the Zebu breed were associated to the higher risk of herd infection by leptospiras.

• Leptospirosis Leptospirose

Abstract in Portuguese:

ABSTRACT.- Figueiredo A.O., Pellegrin A.O., Gonçalves V.S.P., Freitas E.B., Monteiro L.A.R.C., Oliveira J.M. & Osório A.L.A.R. 2009. [Prevalence and risk factors for bovine leptospirosis in Mato Grosso do Sul, Brazil.] Prevalência e fatores de risco para a leptospirose em bovinos do Mato Grosso do Sul. Pesquisa Veterinária Brasileira 29(5):375-381. Programa Mestrado em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil. E-mail: line_figueiredo@yahoo.com.br The prevalence of anti-Leptospira spp. antibodies was estimated for female cattle aged 24 months or older. The sample comprised 178 herds from 22 counties in the state of Mato Grosso do Sul, Brazil. The risk factors associated with the presence of infeccion were investigated. A total of 2,573 blood serum samples were tested against 10 leptospira serovars using the microagglutination test (MAT). Titers of 100 or higher for one or more serovars were detected in 1,801 females (98.8%) from 161 herds (96.5%). Serovar Hardjo (65.6%) was the most frequent, followed by serovar Wolffi (12.3%). These results suggest that bovine leptospirosis is widespread in all the counties under study, with a high prevalence both at the animal and the herd level. Beef farms and the Zebu breed were associated to the higher risk of herd infection by leptospiras.

Abstract in English:

ABSTRACT.- Pierezan F., Rissi D.R., Oliveira Filho J.C., Lucena R.B., Tochetto C., Flores M.M., Rosa F.B & Barros C.S.L. 2009. [Granulomatous enteritis associated with larval cyathostomiasis in horses in Rio Grande do Sul, Brazil.] Enterite granulomatosa associada a larvas de ciatostomíneos em eqüinos no Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(5):382-386. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: claudioslbarros@uol.com.br Five cases of granulomatous enteritis associated with cyathostomes are described in horses from three farms in Rio Grande do Sul, Brazil. The cases occurred from January 1999 to December 2007. The clinical course in two cases in which clinical follow-up was available was 10-14 days. Clinical signs presented by two horses were similar and included diarrhea (3/3), weight loss (2/3), pyrexia (1/3), tachycardia (1/3), and tachypnea (1/3). Gross changes consisted of thickening of the wall of large colon and cecum by edema and the occurrence of numerous 1-4mm, dark-tan, slightly raised multifocal pinpoints disseminated throughout the mucosa. Up on the incision of these pinpoints, brown-reddish, small (1-2mm) nematode larvae emerged. Large numbers of parasites with morphology compatible with small strongyles were observed in the lumina of large colon and cecum or adhered to the mucosae of these organs. Histologically, multiple granulomas were seen in the mucosa and submucosa of large colon and cecum. These granulomas consisted of moderate to severe inflammatory infiltrate of macrophages, epithelioid macrophages, and eosinophils surrounded by fewer lymphocytes and plasma cells. In the center of these granulomas, transversal cut sections of parasites with morphology compatible with cyathostomes larvae were observed. Additionally, there was edema and moderate to marked lymphohistioplasmacytic and eosinophilic inflammatory infiltrate throughout the mucosa and submucosa of the large colon and cecum; necrosis and lymphohistiocytic proliferation in the submucosal lymphoid follicles, with hyperplasia of goblet cells in the epithelial lining of these organs were also observed. The diagnosis of granulomatous enteritis associated with larval cyathostomiasis was made based on epidemiological, clinical, and gross findings which were confirmed by histopathology.

• Diseases of horses strongylus cyathostomes Doenças de eqüinos estrôngilos ciastotomíneos

Abstract in Portuguese:

ABSTRACT.- Pierezan F., Rissi D.R., Oliveira Filho J.C., Lucena R.B., Tochetto C., Flores M.M., Rosa F.B & Barros C.S.L. 2009. [Granulomatous enteritis associated with larval cyathostomiasis in horses in Rio Grande do Sul, Brazil.] Enterite granulomatosa associada a larvas de ciatostomíneos em eqüinos no Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(5):382-386. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: claudioslbarros@uol.com.br Five cases of granulomatous enteritis associated with cyathostomes are described in horses from three farms in Rio Grande do Sul, Brazil. The cases occurred from January 1999 to December 2007. The clinical course in two cases in which clinical follow-up was available was 10-14 days. Clinical signs presented by two horses were similar and included diarrhea (3/3), weight loss (2/3), pyrexia (1/3), tachycardia (1/3), and tachypnea (1/3). Gross changes consisted of thickening of the wall of large colon and cecum by edema and the occurrence of numerous 1-4mm, dark-tan, slightly raised multifocal pinpoints disseminated throughout the mucosa. Up on the incision of these pinpoints, brown-reddish, small (1-2mm) nematode larvae emerged. Large numbers of parasites with morphology compatible with small strongyles were observed in the lumina of large colon and cecum or adhered to the mucosae of these organs. Histologically, multiple granulomas were seen in the mucosa and submucosa of large colon and cecum. These granulomas consisted of moderate to severe inflammatory infiltrate of macrophages, epithelioid macrophages, and eosinophils surrounded by fewer lymphocytes and plasma cells. In the center of these granulomas, transversal cut sections of parasites with morphology compatible with cyathostomes larvae were observed. Additionally, there was edema and moderate to marked lymphohistioplasmacytic and eosinophilic inflammatory infiltrate throughout the mucosa and submucosa of the large colon and cecum; necrosis and lymphohistiocytic proliferation in the submucosal lymphoid follicles, with hyperplasia of goblet cells in the epithelial lining of these organs were also observed. The diagnosis of granulomatous enteritis associated with larval cyathostomiasis was made based on epidemiological, clinical, and gross findings which were confirmed by histopathology.

Abstract in English:

ABSTRACT.- Barbosa J.D., Silveira J.A.S, Albernaz T.T., Silva N.S., Belo Reis A.S., Oliveira C.M.C., Riet- Correa G. & Duarte M.D. 2009 [Cutaneous lesions of the legs caused by the thorns of Mimosa pudica (Leg. Mimosoideae) in cattle and sheep in the State of Pará.] Lesões de pele causadas por Mimosa pudica (Leg. Mimosoideae) nos membros de bovinos e ovinos no Estado do Pará. Pesquisa Veterinária Brasileira 29(5):435-438. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition of cattle and sheep characterized by ulcerous and granulomatous skin lesions of the legs was studied. Epidemiological data and the nature of the lesions indicate that they are caused by the thorns of Mimosa pudica (Leg. Mimosoideae). The disease was observed only in pastures largely infested by the plant, and animals recover quickly when moved from them.

• Mimosa pudica Leguminosae Mimosoideae traumatic lesions malícia lesões traumáticas

Abstract in Portuguese:

ABSTRACT.- Barbosa J.D., Silveira J.A.S, Albernaz T.T., Silva N.S., Belo Reis A.S., Oliveira C.M.C., Riet- Correa G. & Duarte M.D. 2009 [Cutaneous lesions of the legs caused by the thorns of Mimosa pudica (Leg. Mimosoideae) in cattle and sheep in the State of Pará.] Lesões de pele causadas por Mimosa pudica (Leg. Mimosoideae) nos membros de bovinos e ovinos no Estado do Pará. Pesquisa Veterinária Brasileira 29(5):435-438. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition of cattle and sheep characterized by ulcerous and granulomatous skin lesions of the legs was studied. Epidemiological data and the nature of the lesions indicate that they are caused by the thorns of Mimosa pudica (Leg. Mimosoideae). The disease was observed only in pastures largely infested by the plant, and animals recover quickly when moved from them.

Abstract in English:

ABSTRACT.- Barbosa J.D., Albernaz T.T., Riet-Correa G, Cerqueira V.D., Soares S.O., Campos K.F., Oliveira C.M.C. & Duarte M.D. 2009. [Angular cheilitis in horses associated with ingestion of Panicum maximum.] Queilite angular traumática em eqüinos associada à ingestão de Panicum maximum. Pesquisa Veterinária Brasileira 29(5):428-430. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition with enlargement of the oral cleft in horses was studied. The enlargement of varied extension was uni or bilateral. The cheek mucosa of the labial commissure showed slight erosions. During chewing there was loss of small amounts of grass and saliva through the oral cleft. The affected horses were in good nutritional condition. Histopathological studies of tissues obtained by biopsia, revealed a superficial epidermitis. The pastures consisted of Panicum maximum grass (varieties Tanzânia, Mombaça, Tobiatã and Colonião) which was mature, tall, lignified, with leaves of cutting edges. Based on epidemiological, clinical and histopathological data, it was concluded that the lesions were caused by the hard grass, favored by the way horses pull the tall grass and chew it.

• Cheilitis Panicum maximum diseases of horses

Abstract in Portuguese:

ABSTRACT.- Barbosa J.D., Albernaz T.T., Riet-Correa G, Cerqueira V.D., Soares S.O., Campos K.F., Oliveira C.M.C. & Duarte M.D. 2009. [Angular cheilitis in horses associated with ingestion of Panicum maximum.] Queilite angular traumática em eqüinos associada à ingestão de Panicum maximum. Pesquisa Veterinária Brasileira 29(5):428-430. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition with enlargement of the oral cleft in horses was studied. The enlargement of varied extension was uni or bilateral. The cheek mucosa of the labial commissure showed slight erosions. During chewing there was loss of small amounts of grass and saliva through the oral cleft. The affected horses were in good nutritional condition. Histopathological studies of tissues obtained by biopsia, revealed a superficial epidermitis. The pastures consisted of Panicum maximum grass (varieties Tanzânia, Mombaça, Tobiatã and Colonião) which was mature, tall, lignified, with leaves of cutting edges. Based on epidemiological, clinical and histopathological data, it was concluded that the lesions were caused by the hard grass, favored by the way horses pull the tall grass and chew it.

Abstract in English:

ABSTRACT.- Beserra P.S., Sales G.A., Santana E.J.M., Miranda S.A., Brito A.B., Nickolak E., Freitas V.V. & Domingues S.F.S. 2009. [B-Mode Ultrasound biometry of the ocular globe related with fronto-occiptal and bizigomatic diameters in Canis familiaris.] Relação entre a biometria ultra-sonográfica em modo B do globo ocular e os diâmetros fronto occiptal e bizigomático em Canis familiaris. Pesquisa Veterinária Brasileira 29(4):286-290. Laboratório de Biologia e Medicina de Animais da Amazônia, Medicina Veterinária, Universidade Federal do Pará, Rua Hernane Lameira n° 556, Castanhal, PA 68743-050, Brazil. E-mail: shfarha@ufpa.br In the experiment 31 healthy mongrel dogs, 8 months to 7 years of age, were used, 10 males and 21 females, weighing 1.5-28 kg. Initially, fronto-occipetal (FOD) and bizigomatic (BZD) diameters were measured using a caliper. The ophthalmologic transpalpebral B-mode ultrasonography (US) was performed to measure the internal ocular structures, as follows: The cornea thickness (D1), distance between cornea and anterior lens capsule (D2), distance between cornea and posterior lens capsule (D3), lens thickness (D4), lens diameter (D5), lens area (D6), distance between posterior lens capsule and retina (D7), distance between anterior lens capsule and retina (D8), and the distance between cornea and retina (D9). Except for D4, there were effect of FOD and BZD on the measures of the internal structures of GO. The Lineal Regression Analysis between the measures of the internal oculars structures and DFO and DBZ were significant for D1, D2, D3, D4, D5, D6, D7, D8 and D9.

• B-mode ultrasonography ocular biometry bizigomatic diameter fronto-occiptal diameter dogs

Abstract in Portuguese:

ABSTRACT.- Beserra P.S., Sales G.A., Santana E.J.M., Miranda S.A., Brito A.B., Nickolak E., Freitas V.V. & Domingues S.F.S. 2009. [B-Mode Ultrasound biometry of the ocular globe related with fronto-occiptal and bizigomatic diameters in Canis familiaris.] Relação entre a biometria ultra-sonográfica em modo B do globo ocular e os diâmetros fronto occiptal e bizigomático em Canis familiaris. Pesquisa Veterinária Brasileira 29(4):286-290. Laboratório de Biologia e Medicina de Animais da Amazônia, Medicina Veterinária, Universidade Federal do Pará, Rua Hernane Lameira n° 556, Castanhal, PA 68743-050, Brazil. E-mail: shfarha@ufpa.br In the experiment 31 healthy mongrel dogs, 8 months to 7 years of age, were used, 10 males and 21 females, weighing 1.5-28 kg. Initially, fronto-occipetal (FOD) and bizigomatic (BZD) diameters were measured using a caliper. The ophthalmologic transpalpebral B-mode ultrasonography (US) was performed to measure the internal ocular structures, as follows: The cornea thickness (D1), distance between cornea and anterior lens capsule (D2), distance between cornea and posterior lens capsule (D3), lens thickness (D4), lens diameter (D5), lens area (D6), distance between posterior lens capsule and retina (D7), distance between anterior lens capsule and retina (D8), and the distance between cornea and retina (D9). Except for D4, there were effect of FOD and BZD on the measures of the internal structures of GO. The Lineal Regression Analysis between the measures of the internal oculars structures and DFO and DBZ were significant for D1, D2, D3, D4, D5, D6, D7, D8 and D9.