PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Costa M.C., Flaiban K.K.M.C., Coneglian M.M., Feitosa F.L.F., Balarin M.R.S. & Lisbôa J.A.N. 2008. [Passive transfer of immunity in Nelore and Limousin calves and serum proteinogram in the first four months of life.] Transferência de imunidade passiva em bezerros das raças Nelore e Limousin e proteinograma sérico nos primeiros quatro meses de vida. Pesquisa Veterinária Brasileira 28(9):410-416. Departamento de Clínicas Veterinárias, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Campus Universitário, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: janlisboa@uel.br To study the passive transfer of immunity, 90 healthy calves, 45 Nelore and 45 Limousin, were grouped (n=15) according to their dam’s parity: first, second or third or more calvings. Blood samples were draw from each calf with 24 to 36 hours of life and 15, 30, 60, 90, and 120 days. The total serum (TSP) and plasma (TPP) proteins, gamaglutamiltransferase activity (GGT), serum albumin, alpha, beta and gammaglobulin by electrophoresis in agarose gel and IgG estimated by the zinc sulphate turbidity test were determined. Two-way-ANOVA was used to compare the data in the first age. The age behaviour was analysed through repeated measures ANOVA. Correlations were established between the variables. The passive transfer of immunity was successful in both breeds and the parity of the dam had no effect on the calves’ serum gammaglobulin concentration. The levels of gammaglobulins were higher at the end of the first day of life, and decreased until 60 days. From there on, the increase due to the active production of antibodies was precocious in taurine calves and slower in Zebu calves. At the end of the first day of life, the gammaglobulin was correlated with IgG (r=0,859), TPP (r=0,807), TSP (r=0,811) and GGT (r=0,399). The variation of serum proteins followed the normal pattern throughout the first four months of life, with little differences between taurine and Zebu calves.

• Calves Zebu taurine serum proteins immunoglobulins

Abstract in Portuguese:

ABSTRACT.- Costa M.C., Flaiban K.K.M.C., Coneglian M.M., Feitosa F.L.F., Balarin M.R.S. & Lisbôa J.A.N. 2008. [Passive transfer of immunity in Nelore and Limousin calves and serum proteinogram in the first four months of life.] Transferência de imunidade passiva em bezerros das raças Nelore e Limousin e proteinograma sérico nos primeiros quatro meses de vida. Pesquisa Veterinária Brasileira 28(9):410-416. Departamento de Clínicas Veterinárias, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Campus Universitário, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: janlisboa@uel.br To study the passive transfer of immunity, 90 healthy calves, 45 Nelore and 45 Limousin, were grouped (n=15) according to their dam’s parity: first, second or third or more calvings. Blood samples were draw from each calf with 24 to 36 hours of life and 15, 30, 60, 90, and 120 days. The total serum (TSP) and plasma (TPP) proteins, gamaglutamiltransferase activity (GGT), serum albumin, alpha, beta and gammaglobulin by electrophoresis in agarose gel and IgG estimated by the zinc sulphate turbidity test were determined. Two-way-ANOVA was used to compare the data in the first age. The age behaviour was analysed through repeated measures ANOVA. Correlations were established between the variables. The passive transfer of immunity was successful in both breeds and the parity of the dam had no effect on the calves’ serum gammaglobulin concentration. The levels of gammaglobulins were higher at the end of the first day of life, and decreased until 60 days. From there on, the increase due to the active production of antibodies was precocious in taurine calves and slower in Zebu calves. At the end of the first day of life, the gammaglobulin was correlated with IgG (r=0,859), TPP (r=0,807), TSP (r=0,811) and GGT (r=0,399). The variation of serum proteins followed the normal pattern throughout the first four months of life, with little differences between taurine and Zebu calves.

Abstract in English:

ABSTRACT.- Costa M.C., Flaiban K.K.M.C., Coneglian M.M., Dognani R., Vettorato E.D., Balarin M.R.S. & Lisbôa J.A.N. 2008. [Neutrophil oxidative burst in Nelore and Limousin calves in the first four months of life.] Metabolismo oxidativo dos neutrófilos de bezerros das raças Nelore e Limousin nos primeiros quatro meses de vida. Pesquisa Veterinária Brasileira 28(9):431-436. Departamento de Clínicas Veterinárias, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Campus Universitário, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: janlisboa@uel.br To study some defence mechanisms of beef calves kept on range conditions, 90 healthy calves, 45 Nelore and 45 Limousin, were bled at 24 to 36 hours of life, 15, 30, 60, 90 and, 120 days. Leukogram and neutrophil oxidative burst through the non-stimulated and the stimulated NBT tests were determined. The parasitemia with Anaplasma marginale, Babesia bigemina and B. bovis was investigated. Repeated measure ANOVA was used to analyse the age effect on leukocyte counts. Variation of positive neutrophil throughout the age was analysed through Krushkal-Wallis test. Comparisons among breeds were done using the Mann-Whithney test. The white blood cell count increased with age in both breeds, with a decrease in neutrophil and an increase in lymphocyte counts. The neutrophil lymphocyte ratio had been inverted before 15 days of life. The neutrophil oxidative burst was less effective in the newborn calves increasing with age. Limousin calves’ neutrophils had higher capacity to reduce NBT in advanced ages. The observed differences between taurine and Zebu calves had no effects on calves’ health and could be atributed to Anaplasma marginale natural and asynptomatic infection.

• Beef calf Zebu taurine NBT Anaplasma marginale

Abstract in Portuguese:

ABSTRACT.- Costa M.C., Flaiban K.K.M.C., Coneglian M.M., Dognani R., Vettorato E.D., Balarin M.R.S. & Lisbôa J.A.N. 2008. [Neutrophil oxidative burst in Nelore and Limousin calves in the first four months of life.] Metabolismo oxidativo dos neutrófilos de bezerros das raças Nelore e Limousin nos primeiros quatro meses de vida. Pesquisa Veterinária Brasileira 28(9):431-436. Departamento de Clínicas Veterinárias, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Campus Universitário, Cx. Postal 6001, Londrina, PR 86051-990, Brazil. E-mail: janlisboa@uel.br To study some defence mechanisms of beef calves kept on range conditions, 90 healthy calves, 45 Nelore and 45 Limousin, were bled at 24 to 36 hours of life, 15, 30, 60, 90 and, 120 days. Leukogram and neutrophil oxidative burst through the non-stimulated and the stimulated NBT tests were determined. The parasitemia with Anaplasma marginale, Babesia bigemina and B. bovis was investigated. Repeated measure ANOVA was used to analyse the age effect on leukocyte counts. Variation of positive neutrophil throughout the age was analysed through Krushkal-Wallis test. Comparisons among breeds were done using the Mann-Whithney test. The white blood cell count increased with age in both breeds, with a decrease in neutrophil and an increase in lymphocyte counts. The neutrophil lymphocyte ratio had been inverted before 15 days of life. The neutrophil oxidative burst was less effective in the newborn calves increasing with age. Limousin calves’ neutrophils had higher capacity to reduce NBT in advanced ages. The observed differences between taurine and Zebu calves had no effects on calves’ health and could be atributed to Anaplasma marginale natural and asynptomatic infection.