Resultado da pesquisa (2)
Termo utilizado na pesquisa antibiotic resistance genes
#1 - Investigation of antimicrobial resistance genes in Staphylococcus aureus from bovine mastitis milk in Southwest Turkey
Abstract in English:
One of the most important pathogens of bovine mastitis, Staphylococcus aureus, often shows antibiotic resistance due to the widespread use of antibiotics in dairy herds. Here, we analyzed S. aureus isolates recovered from milk samples of mastitis-affected cows from various dairy farms for the presence of specific antibiotic resistance genes (mecA, blaZ, aacA-aphD, tetK, tetM, vanA, cfr, fusB, and ileS). To isolate and identify S. aureus, milk was inoculated onto Baird-Parker agar plates. The thermonuclease gene (nuc) was used for molecular identification of each S. aureus isolate. The chosen resistance genes were found in 14 verified isolates. The blaZ and tetM genes were found in all isolates (100%), whereas the aacA-aphD, mecA, and cfr genes were found in 42.9%, 14.3%, and 7.1% of the isolates, respectively. TetK, vanA, fusB, and ileS genes were not found in any of the isolates. Every isolate carried at least blaZ and tetM, demonstrating the widespread co-carriage of several genes. The findings show that S. aureus from bovine mastitis in this area exhibits broad genotypic resistance to β-lactams and tetracyclines, and emphasize the need for ongoing regional surveillance of resistance determinants.
Abstract in Portuguese:
Staphylococcus aureus, um dos patógenos mais importantes da mastite bovina, frequentemente apresenta resistência aos antibióticos devido ao uso generalizado de antibióticos em rebanhos leiteiros. Analisamos isolados de S. aureus recuperados de amostras de leite de vacas afetadas por mastite de várias fazendas leiteiras para a presença de genes específicos de resistência aos antibióticos (mecA, blaZ, aacA-aphD, tetK, tetM, vanA, cfr, fusB, e ileS). Para isolar e identificar S. aureus, o leite foi inoculado em placas de ágar Baird-Parker. O gene termonuclease (nuc) foi usado para a identificação molecular de cada isolado de S. aureus. Os genes de resistência escolhidos foram encontrados em 14 isolados verificados. Os genes blaZ e tetM foram encontrados em todos os isolados (100%), enquanto os genes aacA-aphD, mecA e cfr foram encontrados em 42,9%, 14,3% e 7,1% dos isolados, respectivamente. Os genes TetK, vanA, fusB e ileS não foram encontrados em nenhum dos isolados. Todos os isolados carregavam pelo menos blaZ e tetM, demonstrando o transporte conjunto generalizado de vários genes. Os resultados mostram que S. aureus da mastite bovina nesta região tem ampla resistência genotípica aos β-lactâmicos e tetraciclinas, e enfatizam a necessidade de vigilância regional contínua dos determinantes de resistência
#2 - Detection of virulence genes, antibiotic resistance genes and antibiotic resistance of Escherichia coli isolated from diarrheic lambs in Anhui Province, China
Abstract in English:
Lamb diarrhea seriously restricts the development of the sheep industry. Infectious pathogens often cause diarrhea, and E. coli isolates are highly distributed in infectious diarrhea. The study aimed to investigate the prevalence of pathogenic E. coli in diarrhea lambs and determine the distribution of virulence genes, antibiotic resistance genes, and antibiotic resistance. One hundred seventy-eight E. coli isolates were isolated from the feces of 204 diarrhea lambs. The virulence genes mdh, hlyF, iss, ompA, fimC, iucD, st, lt, stx1, stx2, and antibiotic resistance genes including tetA, tetB, aac (6′)-II, blaCMY-2, qnr, aadA1, sul1, blaTEM, blaCTX-M were detected by polymerase chain reaction (PCR). Antibiotic resistance was determined by Kirby-Bauer Disc Diffusion method. There were 109 (61.24%, 109/178) enterotoxigenic E. coli (ETEC), 119 (66.85%, 119/178) Shiga toxin-producing E. coli (STEC), and 95 (53.37%, 95/178) hybrid STEC/ETEC isolates. The highest prevalent virulence genes were ompA (80.90%, 144/178) and fimC (67.98%, 121/178), and the lowest was iucD (8.99%, 16/178). The most commonly detected antibiotic resistance genes were tetA/tetB (92.70%, 165/178), qnr (75.84%, 135/178), and sul1 (62.92%, 112/178), no blaTEM or blaCTX-M genes were detected. All isolates had high antibiotic resistance to lincomycin (96.63%, 172/178), tetracycline (88.76%, 158/178), and co-trimoxazole (80.34%, 143/178), and the multidrug resistant (MDR) rate reached 93.82% (167/178). The high prevalence of ETEC and STEC indicates that E. coli is one of the critical pathogenic agents leading to diarrhea in lambs in the region, and its high antibiotic resistance, especially MDR, should be brought to our attention.
Abstract in Portuguese:
Lamb diarrhea seriously restricts the development of the sheep industry. Infectious pathogens often cause diarrhea, and E. coli isolates are highly distributed in infectious diarrhea. The study aimed to investigate the prevalence of pathogenic E. coli in diarrhea lambs and determine the distribution of virulence genes, antibiotic resistance genes, and antibiotic resistance. One hundred seventy-eight E. coli isolates were isolated from the feces of 204 diarrhea lambs. The virulence genes mdh, hlyF, iss, ompA, fimC, iucD, st, lt, stx1, stx2, and antibiotic resistance genes including tetA, tetB, aac (6′)-II, blaCMY-2, qnr, aadA1, sul1, blaTEM, blaCTX-M were detected by polymerase chain reaction (PCR). Antibiotic resistance was determined by Kirby-Bauer Disc Diffusion method. There were 109 (61.24%, 109/178) enterotoxigenic E. coli (ETEC), 119 (66.85%, 119/178) Shiga toxin-producing E. coli (STEC), and 95 (53.37%, 95/178) hybrid STEC/ETEC isolates. The highest prevalent virulence genes were ompA (80.90%, 144/178) and fimC (67.98%, 121/178), and the lowest was iucD (8.99%, 16/178). The most commonly detected antibiotic resistance genes were tetA/tetB (92.70%, 165/178), qnr (75.84%, 135/178), and sul1 (62.92%, 112/178), no blaTEM or blaCTX-M genes were detected. All isolates had high antibiotic resistance to lincomycin (96.63%, 172/178), tetracycline (88.76%, 158/178), and co-trimoxazole (80.34%, 143/178), and the multidrug resistant (MDR) rate reached 93.82% (167/178). The high prevalence of ETEC and STEC indicates that E. coli is one of the critical pathogenic agents leading to diarrhea in lambs in the region, and its high antibiotic resistance, especially MDR, should be brought to our attention.
