Abstract in English:
ABSTRACT.- Ferro D.G. & Gioso M.A. 2009. [Clinical study of effectiveness of an Anorganic Bone Matrix and Cell-Binding Peptide (ABM/P-15), PepGen-P15®: Application in advanced periodontal lesions of dogs.] Estudo clínico da aplicação de matriz inorgânica de osso associado a peptídeo sintético de adesão celular (MIO/P-15), PepGen P-15®, em lesões periodontais avançadas de cães. Pesquisa Veterinária Brasileira 29(2):109-116. Laboratório de Odontologia Comparada, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: deferro@usp.br
The development of biologic modalities designed to enhance bone regeneration and wound healing of specific periodontal sites continues to be a challenge and has been accomplished through the cell binding activity of Type-I collagen. These have been provided by a synthetic cell biding peptide (P-15), associated to a anorganic bone matrix (ABM) to form ABM/P-15. The aim of this study was to evaluate the attachment loss and periodontal pocket response in teeth after 3 and 6 months with ABM/P-15 graft application. Twenty one dogs from the Veterinary Hospital, University of São Paulo, were anesthetized in order to accomplish periodontal treatment and 132 teeth faces with attachment loss were treated. From these, 36.4% (48 faces) received cell binding peptide and 63.6% (84 faces) compounded the control group that received conventional treatment (muco-gingival flap and root planning). The procedure was documented by intra-oral radiography and all periodontal probings were photographed. After 3 and 6 months, the animals were re-anesthetized in order to accomplish new photography, radiography and periodontal probing exams. The 48 attachment loss faces that received graft material exhibited 40% of regeneration rate after 6 months. The control faces did not change their attachment level. The palatal face presented the better regeneration rates (40%) and the canines and molars teeth showed the better responses (57.14% and 65%, respectively). There was no post-surgical infection related to absence of oral home care. It can be concluded that ABM/P-15 helps a more rapidly periodontal structure re-attachment and regeneration, including alveolar bone. Its application was easy and practical, and the post-surgical complications incidence was low. Nevertheless, more work is necessary to evaluate the amount and the quality of formed bone and periodontal ligament.
Abstract in Portuguese:
ABSTRACT.- Ferro D.G. & Gioso M.A. 2009. [Clinical study of effectiveness of an Anorganic Bone Matrix and Cell-Binding Peptide (ABM/P-15), PepGen-P15®: Application in advanced periodontal lesions of dogs.] Estudo clínico da aplicação de matriz inorgânica de osso associado a peptídeo sintético de adesão celular (MIO/P-15), PepGen P-15®, em lesões periodontais avançadas de cães. Pesquisa Veterinária Brasileira 29(2):109-116. Laboratório de Odontologia Comparada, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: deferro@usp.br
The development of biologic modalities designed to enhance bone regeneration and wound healing of specific periodontal sites continues to be a challenge and has been accomplished through the cell binding activity of Type-I collagen. These have been provided by a synthetic cell biding peptide (P-15), associated to a anorganic bone matrix (ABM) to form ABM/P-15. The aim of this study was to evaluate the attachment loss and periodontal pocket response in teeth after 3 and 6 months with ABM/P-15 graft application. Twenty one dogs from the Veterinary Hospital, University of São Paulo, were anesthetized in order to accomplish periodontal treatment and 132 teeth faces with attachment loss were treated. From these, 36.4% (48 faces) received cell binding peptide and 63.6% (84 faces) compounded the control group that received conventional treatment (muco-gingival flap and root planning). The procedure was documented by intra-oral radiography and all periodontal probings were photographed. After 3 and 6 months, the animals were re-anesthetized in order to accomplish new photography, radiography and periodontal probing exams. The 48 attachment loss faces that received graft material exhibited 40% of regeneration rate after 6 months. The control faces did not change their attachment level. The palatal face presented the better regeneration rates (40%) and the canines and molars teeth showed the better responses (57.14% and 65%, respectively). There was no post-surgical infection related to absence of oral home care. It can be concluded that ABM/P-15 helps a more rapidly periodontal structure re-attachment and regeneration, including alveolar bone. Its application was easy and practical, and the post-surgical complications incidence was low. Nevertheless, more work is necessary to evaluate the amount and the quality of formed bone and periodontal ligament.
Abstract in English:
ABSTRACT.- Rezende R.S., Coelho H.E, Kamimura R., Severino R.S, Oliveira, P.C.L., Medeiros A.A. & Magalhães A.O.C. 2009. [Microscopic analysis of the left ventricular myocardium in positive serum dogs to distemper disease.] Análise microscópica do miocárdio ventricular esquerdo em cães soropositivos para cinomose. Pesquisa Veterinária Brasileira 29(2):117-119. Instituto de Estudos Avançados em Veterinária José Caetano Borges, Universidade de Uberaba, Av. do Tutunas 720, Uberaba, MG 38061-500, Brazil. E-mail: rezendehvu@hotmail.com
Classified pertaining to the genus Morbillivirus of the Paramyxoviridae family, the canine distemper virus is a RNA single-stranded virus with negative polarity and causes a multisystemic disease, serious and highly contagious for dogs and wild carnivores, with a high mortality rate in non-vaccinated animals or with vaccine fails. With the objective to evaluate heart histopathological alterations, particularly in the left ventricular myocardium, in dogs naturally infected with canine distemper virus, 35 dogs, males and females of different ages, were studied. All the 35 samples sent to the Veterinary Hospital of Uberaba were serum-positive for distemper (immunoassay technique in solid phase) and had in the left ventricular myocardium the following histopathologic alterations: myocarditis, hyalin degeneration, hyperemia and hemorrhage, in 42.8% (15/35), 31.4% (11/35), 14.3% (5/35) and 11.4% (4/35), respectively. Having carried out the Qui-Quadrado test with a significancy level of 0.05, it can be concluded that there is a high correlation (p=0.02) between the infected animals with canine distemper virus and histopathological alterations found in the left ventricular myocardium.
Abstract in Portuguese:
ABSTRACT.- Rezende R.S., Coelho H.E, Kamimura R., Severino R.S, Oliveira, P.C.L., Medeiros A.A. & Magalhães A.O.C. 2009. [Microscopic analysis of the left ventricular myocardium in positive serum dogs to distemper disease.] Análise microscópica do miocárdio ventricular esquerdo em cães soropositivos para cinomose. Pesquisa Veterinária Brasileira 29(2):117-119. Instituto de Estudos Avançados em Veterinária José Caetano Borges, Universidade de Uberaba, Av. do Tutunas 720, Uberaba, MG 38061-500, Brazil. E-mail: rezendehvu@hotmail.com
Classified pertaining to the genus Morbillivirus of the Paramyxoviridae family, the canine distemper virus is a RNA single-stranded virus with negative polarity and causes a multisystemic disease, serious and highly contagious for dogs and wild carnivores, with a high mortality rate in non-vaccinated animals or with vaccine fails. With the objective to evaluate heart histopathological alterations, particularly in the left ventricular myocardium, in dogs naturally infected with canine distemper virus, 35 dogs, males and females of different ages, were studied. All the 35 samples sent to the Veterinary Hospital of Uberaba were serum-positive for distemper (immunoassay technique in solid phase) and had in the left ventricular myocardium the following histopathologic alterations: myocarditis, hyalin degeneration, hyperemia and hemorrhage, in 42.8% (15/35), 31.4% (11/35), 14.3% (5/35) and 11.4% (4/35), respectively. Having carried out the Qui-Quadrado test with a significancy level of 0.05, it can be concluded that there is a high correlation (p=0.02) between the infected animals with canine distemper virus and histopathological alterations found in the left ventricular myocardium.
Abstract in English:
ABSTRACT.- Garcia D.C., Stopiglia A.J., Mingrone L.E. & Fantoni D.T. 2009. [Clinical evaluation of dogs submitted to circulatory arrest for different periods of time by “Inflow Occlusion”.] Avaliação clínica de cães submetidos à parada circulatória total por diferentes períodos de tempo através da técnica de “Inflow Occlusion”. Pesquisa Veterinária Brasileira 29(2):125-130. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: danielcgarcia@hotmail.com
“Inflow Occlusion” technique can be used in heart surgeries when heart is required to be opened just for few minutes, to allow quick repairs. However, circulatory arrest, event occasioned by this technique, may produce serious metabolic and neurological consequences to the patient. In this study, 12 mongrel dogs were used, divided into two groups, A and B, which were submitted to 7 and 8 minutes of total circulatory arrest, respectively, using “Inflow Occlusion” technique. Normothermia was tried during surgical procedures. Clinical and behavior evaluation were performed after surgery to both groups, and biochemical data were collected to compare pre and post-operatory moments. There were two transoperatory deaths in Group B. Transitory clinical problems were observed in group A until moment M7 (48 hours after surgery), and in Group B these problems were more intense and seen even after M7; and permanent blindness in one animal of Group B was present during all follow up period. Despite all alterations found during the study, it might be safe to use “Inflow Occlusion” technique for periods up to 7 minutes, however, it is contra-indicated for longer periods.
Abstract in Portuguese:
ABSTRACT.- Garcia D.C., Stopiglia A.J., Mingrone L.E. & Fantoni D.T. 2009. [Clinical evaluation of dogs submitted to circulatory arrest for different periods of time by “Inflow Occlusion”.] Avaliação clínica de cães submetidos à parada circulatória total por diferentes períodos de tempo através da técnica de “Inflow Occlusion”. Pesquisa Veterinária Brasileira 29(2):125-130. Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: danielcgarcia@hotmail.com
“Inflow Occlusion” technique can be used in heart surgeries when heart is required to be opened just for few minutes, to allow quick repairs. However, circulatory arrest, event occasioned by this technique, may produce serious metabolic and neurological consequences to the patient. In this study, 12 mongrel dogs were used, divided into two groups, A and B, which were submitted to 7 and 8 minutes of total circulatory arrest, respectively, using “Inflow Occlusion” technique. Normothermia was tried during surgical procedures. Clinical and behavior evaluation were performed after surgery to both groups, and biochemical data were collected to compare pre and post-operatory moments. There were two transoperatory deaths in Group B. Transitory clinical problems were observed in group A until moment M7 (48 hours after surgery), and in Group B these problems were more intense and seen even after M7; and permanent blindness in one animal of Group B was present during all follow up period. Despite all alterations found during the study, it might be safe to use “Inflow Occlusion” technique for periods up to 7 minutes, however, it is contra-indicated for longer periods.
Abstract in English:
Abstract.- Oliveira E.C., Pescador C.A., Sonne L., Pavarini S.P., Santos A.S., Corbellini L.G. & Driemeier D. 2009. [Immunohistochemical analysis of dogs infected naturally by canine parvovirus.] Análise imuno-histoquímica de cães naturalmente infectados pelo parvovírus canino. Pesquisa Veterinária Brasileira 29(2):131-136. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br
Ninety-six dogs with gross lesions suggestive of canine parvovirus infection were selected and necropsied in the Faculty of Veterinary Medicine, Universidade Federal do Rio Grande do Sul, between March 2005 and November 2006. The main gross lesions were enlargement of the Peyer’s patches in the small intestine and hyperemia in the intestinal mucosa and serosa. Microscopically, the small intestine showed necrotizing enteritis in 77% (74/96) of the dogs examined. However, in 17.7% of the histological evaluation in the small intestine were damaged due to autolytic changes making it difficult to obtain an appropriate interpretation. The immunohistochemistry test was performed in tissues of small intestine, mesenteric lymph nodes, thymus, spleen, tonsils, tongue, and bone marrow in all the 96 selected cases. Parvovirus antigen was detected in 91.6% (88/96) of the dogs necropsied. The best result of the IHC test was seen in samples of small intestine which was positive in 77% (74/96) of the cases. The statistical analysis (Fisher test) showed a weak association between intestinal autolysis and positive result of the IHC test. The chance of the autolysed intestine showing a positive result in the immunohistochemistry test was 0.33 less (OR=0.33, 95% CI:0.10-1.17) when compared with small intestine not autolysed.
Abstract in Portuguese:
Abstract.- Oliveira E.C., Pescador C.A., Sonne L., Pavarini S.P., Santos A.S., Corbellini L.G. & Driemeier D. 2009. [Immunohistochemical analysis of dogs infected naturally by canine parvovirus.] Análise imuno-histoquímica de cães naturalmente infectados pelo parvovírus canino. Pesquisa Veterinária Brasileira 29(2):131-136. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br
Ninety-six dogs with gross lesions suggestive of canine parvovirus infection were selected and necropsied in the Faculty of Veterinary Medicine, Universidade Federal do Rio Grande do Sul, between March 2005 and November 2006. The main gross lesions were enlargement of the Peyer’s patches in the small intestine and hyperemia in the intestinal mucosa and serosa. Microscopically, the small intestine showed necrotizing enteritis in 77% (74/96) of the dogs examined. However, in 17.7% of the histological evaluation in the small intestine were damaged due to autolytic changes making it difficult to obtain an appropriate interpretation. The immunohistochemistry test was performed in tissues of small intestine, mesenteric lymph nodes, thymus, spleen, tonsils, tongue, and bone marrow in all the 96 selected cases. Parvovirus antigen was detected in 91.6% (88/96) of the dogs necropsied. The best result of the IHC test was seen in samples of small intestine which was positive in 77% (74/96) of the cases. The statistical analysis (Fisher test) showed a weak association between intestinal autolysis and positive result of the IHC test. The chance of the autolysed intestine showing a positive result in the immunohistochemistry test was 0.33 less (OR=0.33, 95% CI:0.10-1.17) when compared with small intestine not autolysed.
Abstract in English:
ABSTRACT.- Andrade J.N.B.M., Stopiglia A.J., Fantoni D.T., Abduch M.A. & Kahvegian M. 2009. Outflow occlusion for circulatory arrest in dogs. Pesquisa Veterinária Brasileira 29(2):137-142. Hospital Veterinário, Universidade de Franca, Av. Dr. Armando Salles Oliveira 201, Franca, SP 14404-600, Brazil. E-mail: jamescardio@terra.com.br
The purpose of this study was to evaluate the possibility of producing circulatory arrest by occlusion of the pulmonary trunk as an alternative to the venous inflow occlusion through the left hemithorax. Eight healthy mongrel dogs were divided in two groups. Group I underwent 4 minutes of outflow occlusion and Group II was submitted to 8 minutes of circulatory arrest. Outflow occlusion was performed through left thoracotomy and pericardiotomy by passing a Rumel tourniquet around the pulmonary trunk. Physical examination, electrocardiography, echocardiography, blood gas analyses, hemodynamic, and oxygen transport variables were obtained before and after the procedure. The dogs from Group I did not have any clinical, electrocardiographic, echocardiographic, or hemo-dynamic abnormalities after anesthetic recover. In the Group II, only one dog survived, which had no clinical, electrocardiographic, or echocardiographic abnormalities. In this last dog, just after releasing the occlusion, it was detected increases in the following parameters: heart rate (HR), systolic, diastolic and mean arterial blood pressure (SAP; DAP; MAP), pulmonary artery pressure (PAP), pulmonary wedge pressure (PWP), central venous pressure (CVP), cardiac output (CO), systolic index (SI), cardiac index (CI), left and right ventricular stroke work (LVSW; RVSW), oxygen delivery index (DO2), oxygen consumption index (VO2), and oxygen extraction (O2 ext). Moreover, the oxygen content of arterial and mixed venous blood (CaO2; CvO2), and the arterial and mixed venous partial pressure of oxygen (PaO2; PvO2) were decreased 5 minutes after circulatory arrest. Outflow occlusion is a feasible surgical procedure for period of 4 minutes of circulatory arrest.
Abstract in Portuguese:
ABSTRACT.- Andrade J.N.B.M., Stopiglia A.J., Fantoni D.T., Abduch M.A. & Kahvegian M. 2009. Outflow occlusion for circulatory arrest in dogs. Pesquisa Veterinária Brasileira 29(2):137-142. Hospital Veterinário, Universidade de Franca, Av. Dr. Armando Salles Oliveira 201, Franca, SP 14404-600, Brazil. E-mail: jamescardio@terra.com.br
The purpose of this study was to evaluate the possibility of producing circulatory arrest by occlusion of the pulmonary trunk as an alternative to the venous inflow occlusion through the left hemithorax. Eight healthy mongrel dogs were divided in two groups. Group I underwent 4 minutes of outflow occlusion and Group II was submitted to 8 minutes of circulatory arrest. Outflow occlusion was performed through left thoracotomy and pericardiotomy by passing a Rumel tourniquet around the pulmonary trunk. Physical examination, electrocardiography, echocardiography, blood gas analyses, hemodynamic, and oxygen transport variables were obtained before and after the procedure. The dogs from Group I did not have any clinical, electrocardiographic, echocardiographic, or hemo-dynamic abnormalities after anesthetic recover. In the Group II, only one dog survived, which had no clinical, electrocardiographic, or echocardiographic abnormalities. In this last dog, just after releasing the occlusion, it was detected increases in the following parameters: heart rate (HR), systolic, diastolic and mean arterial blood pressure (SAP; DAP; MAP), pulmonary artery pressure (PAP), pulmonary wedge pressure (PWP), central venous pressure (CVP), cardiac output (CO), systolic index (SI), cardiac index (CI), left and right ventricular stroke work (LVSW; RVSW), oxygen delivery index (DO2), oxygen consumption index (VO2), and oxygen extraction (O2 ext). Moreover, the oxygen content of arterial and mixed venous blood (CaO2; CvO2), and the arterial and mixed venous partial pressure of oxygen (PaO2; PvO2) were decreased 5 minutes after circulatory arrest. Outflow occlusion is a feasible surgical procedure for period of 4 minutes of circulatory arrest.
Abstract in English:
Abstract.- Sonne L., Oliveira E.C., Pescador C.A., Santos A.S., Pavarini S. P., Carissimi A.S. & Driemeier D. 2009. [Pathologic and immunohistochemistry findings in dogs naturally infected by canine distemper virus.] Achados patológicos e imuno-histoquímicos em cães infectados naturalmente pelo vírus da cinomose canina. Pesquisa Veterinária Brasileira 29(2):143-149. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br
Canine distemper is a viral disease that affects mainly respiratory, gastrointestinal and nervous system. The present study analyzes pathologic and immunohistochemical findings in 54 dogs with canine distemper of a total of 760 canine necropsies performed from July 2006 to October 2007. The gross lesions were characterized by mucopurulent oculonasal discharge, hyperkeratosis of footpads, red and not collapsed lungs, thymic atrophy, watery intestinal content, hyperemia and enlarged Peyer’s patches. The histological findings were characterized by interstitial pneumonia, lymphoid depletion, white matter demyelization, perivascular cuffs and intracytoplasmatic and intranuclear inclusion bodies located in epithelial cells of gastric mucosa, urinary bladder, bronchial, renal pelvis, footpads, eyelid, skin of the ear, tonsil, central nervous system and mononuclear cells in lymph nodes, spleen, and tonsils. Viral antigen was detected by an immunohistochemical procedure using a mouse monoclonal anti-canine distemper antibody. The footpads were the more constantly (67.4% of the cases) immunolabeled tissue, followed by stomach with 62.7%. Immunohistochemistry was demonstrated to be a useful tool for the study of viral antigen distribution in distemper affected dogs as well as it indicated which is the best tissue to be examined in order to confirm a suspected case of canine.
Abstract in Portuguese:
Abstract.- Sonne L., Oliveira E.C., Pescador C.A., Santos A.S., Pavarini S. P., Carissimi A.S. & Driemeier D. 2009. [Pathologic and immunohistochemistry findings in dogs naturally infected by canine distemper virus.] Achados patológicos e imuno-histoquímicos em cães infectados naturalmente pelo vírus da cinomose canina. Pesquisa Veterinária Brasileira 29(2):143-149. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br
Canine distemper is a viral disease that affects mainly respiratory, gastrointestinal and nervous system. The present study analyzes pathologic and immunohistochemical findings in 54 dogs with canine distemper of a total of 760 canine necropsies performed from July 2006 to October 2007. The gross lesions were characterized by mucopurulent oculonasal discharge, hyperkeratosis of footpads, red and not collapsed lungs, thymic atrophy, watery intestinal content, hyperemia and enlarged Peyer’s patches. The histological findings were characterized by interstitial pneumonia, lymphoid depletion, white matter demyelization, perivascular cuffs and intracytoplasmatic and intranuclear inclusion bodies located in epithelial cells of gastric mucosa, urinary bladder, bronchial, renal pelvis, footpads, eyelid, skin of the ear, tonsil, central nervous system and mononuclear cells in lymph nodes, spleen, and tonsils. Viral antigen was detected by an immunohistochemical procedure using a mouse monoclonal anti-canine distemper antibody. The footpads were the more constantly (67.4% of the cases) immunolabeled tissue, followed by stomach with 62.7%. Immunohistochemistry was demonstrated to be a useful tool for the study of viral antigen distribution in distemper affected dogs as well as it indicated which is the best tissue to be examined in order to confirm a suspected case of canine.
Abstract in English:
ABSTRACT.- Souza A.I., Oliveira T.M.F.S., Machado R.Z. & Camacho A.A. 2009. [Seroprevalence of infection by Trypanosoma cruzi in dogs in a rural area of Mato Grosso do Sul, Brazil.] Soroprevalência da infecção por Trypanosoma cruzi em cães de uma área rural do Estado de Mato Grosso do Sul. Pesquisa Veterinária Brasileira 29(2):150-152. Curso de Medicina Veterinária, Universidade para o Desenvolvimento do Estado e da Região do Pantanal, Rua Alexandre Herculano 1400, Jardim Veraneio, Campo Grande, MS 79037-280, Brazil. E-mail: aldaizabel@hotmail.com
Chagas disease is an anthropozoonosis caused by Trypanosoma cruzi and dogs are considered to be one of the main reservoirs of the disease in the South America. This study evaluates the occurrence of the infection caused by T. cruzi in dogs from a rural area of Mato Grosso do Sul, Brazil. Indirect immunofluorescence test (IFI) and enzyme-linked immunosorbent assay (ELISA) was used in 75 dogs living in this area. The antibodies were detected in 45.3% (n=34) and 24.0% (n=18) with IFI and ELISA, respectively. The actual prevalence of the infection was confirmed as 22.7% (n=17) by the criterion of positivity in both tests. The results confirm T. cruzi infection in dogs in the region.
Abstract in Portuguese:
ABSTRACT.- Souza A.I., Oliveira T.M.F.S., Machado R.Z. & Camacho A.A. 2009. [Seroprevalence of infection by Trypanosoma cruzi in dogs in a rural area of Mato Grosso do Sul, Brazil.] Soroprevalência da infecção por Trypanosoma cruzi em cães de uma área rural do Estado de Mato Grosso do Sul. Pesquisa Veterinária Brasileira 29(2):150-152. Curso de Medicina Veterinária, Universidade para o Desenvolvimento do Estado e da Região do Pantanal, Rua Alexandre Herculano 1400, Jardim Veraneio, Campo Grande, MS 79037-280, Brazil. E-mail: aldaizabel@hotmail.com
Chagas disease is an anthropozoonosis caused by Trypanosoma cruzi and dogs are considered to be one of the main reservoirs of the disease in the South America. This study evaluates the occurrence of the infection caused by T. cruzi in dogs from a rural area of Mato Grosso do Sul, Brazil. Indirect immunofluorescence test (IFI) and enzyme-linked immunosorbent assay (ELISA) was used in 75 dogs living in this area. The antibodies were detected in 45.3% (n=34) and 24.0% (n=18) with IFI and ELISA, respectively. The actual prevalence of the infection was confirmed as 22.7% (n=17) by the criterion of positivity in both tests. The results confirm T. cruzi infection in dogs in the region.
Abstract in English:
ABSTRACT.- Pereira I.A., Soares L.C., Coelho S.M.O., Pribul B.R. & Souza M.M.S. 2009. [Susceptibility to azithromycin of bacteria isolated from infectious processes in dogs and cats.] Suscetibilidade à azitromicina de isolados bacterianos de processos infecciosos em cães e gatos. Pesquisa Veterinária Brasileira 29(2):153-156. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. E-mail: miliane@ufrrj.br
The susceptibility pattern to azithromycin of bacterial pathogens from various infectious sites, and the in vitro activity and minimum inhibitory concentration (MIC) of azithromycin were studied. Tests such as disc diffusion and broth microdilution detected respectively 48.6% and 55% of resistant Staphylococcus spp., and 55.3% and 72.7% resistant gram-negative rods. MIC50 for S. aureus was 4.0mg/mL, that for S. intermedius was 1.0mg/mL, for coagulase-negative Staphylococcus e”512mg/mL, and for gram-negative rods 256mg/mL. Fifteen percent (9/60) of oxacilin-resistant, multidrug-resistant and mecA-positive Staphylococcus spp. isolates were also azithromycin resistant. The dissemination of multidrug resistant bacteria points out to the need of antimicrobial evaluation activity in order to select the best indicated drug and thus minimizing therapeutic failures in veterinary practice.
Abstract in Portuguese:
ABSTRACT.- Pereira I.A., Soares L.C., Coelho S.M.O., Pribul B.R. & Souza M.M.S. 2009. [Susceptibility to azithromycin of bacteria isolated from infectious processes in dogs and cats.] Suscetibilidade à azitromicina de isolados bacterianos de processos infecciosos em cães e gatos. Pesquisa Veterinária Brasileira 29(2):153-156. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. E-mail: miliane@ufrrj.br
The susceptibility pattern to azithromycin of bacterial pathogens from various infectious sites, and the in vitro activity and minimum inhibitory concentration (MIC) of azithromycin were studied. Tests such as disc diffusion and broth microdilution detected respectively 48.6% and 55% of resistant Staphylococcus spp., and 55.3% and 72.7% resistant gram-negative rods. MIC50 for S. aureus was 4.0mg/mL, that for S. intermedius was 1.0mg/mL, for coagulase-negative Staphylococcus e”512mg/mL, and for gram-negative rods 256mg/mL. Fifteen percent (9/60) of oxacilin-resistant, multidrug-resistant and mecA-positive Staphylococcus spp. isolates were also azithromycin resistant. The dissemination of multidrug resistant bacteria points out to the need of antimicrobial evaluation activity in order to select the best indicated drug and thus minimizing therapeutic failures in veterinary practice.
Abstract in English:
ABSTRACT.- Souza T.M., Fighera R.A., Schmidt C., Réquia A.H., Brum J.S., Martins T.B. & Barros C.S.L. 2009. [Prevalence of non-tumorous canine dermatopathies in dogs from the municipality of Santa Maria, Rio Grande do Sul, Brazil (2005-2008).] Prevalência das dermatopatias não-tumorais em cães do município de Santa Maria, Rio Grande do Sul (2005-2008). Pesquisa Veterinária Brasileira 29(2):157-162. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: teitsouza@yahoo.com.br
The current study was aimed at determine the prevalence of non-tumorous canine dermatopathies affecting dogs from the municipality of Santa Maria, Rio Grande do Sul, Brazil. From March 2005 to June de 2008, the authors followed-up canine dermatological cases from two sources: those referred to the Dermatology Sector of the Veterinary Teaching Hospital at the Universidade Federal de Santa Maria and those from a private practice. During this period 480 dogs with dermatological problems were examined; in 393 (81.9%) it was possible to establish a definitive diagnosis and in 87 (18.1%) the diagnosis was inconclusive. Four hundred and twenty four primary diagnosis and 78 secondary diagnosis were performed in the 393 dogs with conclusive diagnosis, totaling 502 diagnosis. The distribution of the diagnosis according to the categories of diagnosed dermatopathies was as follows: Allergic (190/502 [37.8%]), bacterial (103/502 [20.5%]), parasitic (97/502 [19.3%]), environmental (28/502 [5,6%]), mycotic (20/502 [4.0%]), endocrine (13/502 [2.6%]), keratinization disturbances (11/502 [2.2%]), psychogenic (9/502 [1.8%]), acquired alopecias (6/502 [1.2%]), autoimmune (6/502 [1.2%]), inherited (6/502 [1.2%]), pigmentary disturbances (1/502 [0.2%], nutritional (1/502 [0.2%]), and sundry conditions (11/502 [2.2%]). In general, the ten most frequently diagnosed non-tumorous dermatopathies in decreasing order of frequency were: Atopy, flea bite allergic dermatitis, bacterial folliculitis, demodectic mange, deep bacterial folliculitis/furunculosis, sarcoptic mange, myiasis, food allergy, traumatic pyoderma, and Malassezia dermatitis. These 10 conditions together made up approximately for three quarters of all canine skin diseases diagnosed in the current study.
Abstract in Portuguese:
ABSTRACT.- Souza T.M., Fighera R.A., Schmidt C., Réquia A.H., Brum J.S., Martins T.B. & Barros C.S.L. 2009. [Prevalence of non-tumorous canine dermatopathies in dogs from the municipality of Santa Maria, Rio Grande do Sul, Brazil (2005-2008).] Prevalência das dermatopatias não-tumorais em cães do município de Santa Maria, Rio Grande do Sul (2005-2008). Pesquisa Veterinária Brasileira 29(2):157-162. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: teitsouza@yahoo.com.br
The current study was aimed at determine the prevalence of non-tumorous canine dermatopathies affecting dogs from the municipality of Santa Maria, Rio Grande do Sul, Brazil. From March 2005 to June de 2008, the authors followed-up canine dermatological cases from two sources: those referred to the Dermatology Sector of the Veterinary Teaching Hospital at the Universidade Federal de Santa Maria and those from a private practice. During this period 480 dogs with dermatological problems were examined; in 393 (81.9%) it was possible to establish a definitive diagnosis and in 87 (18.1%) the diagnosis was inconclusive. Four hundred and twenty four primary diagnosis and 78 secondary diagnosis were performed in the 393 dogs with conclusive diagnosis, totaling 502 diagnosis. The distribution of the diagnosis according to the categories of diagnosed dermatopathies was as follows: Allergic (190/502 [37.8%]), bacterial (103/502 [20.5%]), parasitic (97/502 [19.3%]), environmental (28/502 [5,6%]), mycotic (20/502 [4.0%]), endocrine (13/502 [2.6%]), keratinization disturbances (11/502 [2.2%]), psychogenic (9/502 [1.8%]), acquired alopecias (6/502 [1.2%]), autoimmune (6/502 [1.2%]), inherited (6/502 [1.2%]), pigmentary disturbances (1/502 [0.2%], nutritional (1/502 [0.2%]), and sundry conditions (11/502 [2.2%]). In general, the ten most frequently diagnosed non-tumorous dermatopathies in decreasing order of frequency were: Atopy, flea bite allergic dermatitis, bacterial folliculitis, demodectic mange, deep bacterial folliculitis/furunculosis, sarcoptic mange, myiasis, food allergy, traumatic pyoderma, and Malassezia dermatitis. These 10 conditions together made up approximately for three quarters of all canine skin diseases diagnosed in the current study.
Abstract in English:
ABSTRACT.- Zuccari D.A.P.C, Castro R., Gavioli A.F., Mancini U.M., Frade C.S., Pivaro L.R., Carmona-Raphe J., Terzian A.C.B., Ruiz C.M., Goloni-Bertollo E.M., Pavarino-Bertelli E.C. & Tajara E.H. 2009. The maspin expression in canine mammary tumors: An immunohistochemical and molecular study. Pesquisa Veterinária Brasileira 29(2):167-173. Centro Regional de Bioterismo, Faculdade de Medicina de São José do Rio Preto, Av. Brigadeiro Faria Lima 5416, São José do Rio Preto, SP 15090-000, Brazil. E-mail: debora.zuccari@famerp.br
The serpin maspin, a tumor suppressor in breast cancer was described as an inhibitor of cell migration and inducer of cell adhesion between the basement membrane and extracellular matrix resulting in inhibition of tumor metastasis. In contrast, overexpression of maspin is correlated with poor prognosis in other types of cancer. Little is known about expression, regulation and function of maspin in canine mammary tumors. It was demonstrated in this study, a loss of maspin expression in malignant canine mammary cells compared with a pool of normal canine mammary tissue, analyzed by quantitative real-time PCR; weak maspin expression in malignant canine mammary tumors were observed by immunohistochemistry. It was also demonstrated that a correlation with nuclear maspin expression and a good prognosis. It is suggested that maspin could be used as a prognostic marker in canine mammary neoplasia.
Abstract in Portuguese:
ABSTRACT.- Zuccari D.A.P.C, Castro R., Gavioli A.F., Mancini U.M., Frade C.S., Pivaro L.R., Carmona-Raphe J., Terzian A.C.B., Ruiz C.M., Goloni-Bertollo E.M., Pavarino-Bertelli E.C. & Tajara E.H. 2009. The maspin expression in canine mammary tumors: An immunohistochemical and molecular study. Pesquisa Veterinária Brasileira 29(2):167-173. Centro Regional de Bioterismo, Faculdade de Medicina de São José do Rio Preto, Av. Brigadeiro Faria Lima 5416, São José do Rio Preto, SP 15090-000, Brazil. E-mail: debora.zuccari@famerp.br
The serpin maspin, a tumor suppressor in breast cancer was described as an inhibitor of cell migration and inducer of cell adhesion between the basement membrane and extracellular matrix resulting in inhibition of tumor metastasis. In contrast, overexpression of maspin is correlated with poor prognosis in other types of cancer. Little is known about expression, regulation and function of maspin in canine mammary tumors. It was demonstrated in this study, a loss of maspin expression in malignant canine mammary cells compared with a pool of normal canine mammary tissue, analyzed by quantitative real-time PCR; weak maspin expression in malignant canine mammary tumors were observed by immunohistochemistry. It was also demonstrated that a correlation with nuclear maspin expression and a good prognosis. It is suggested that maspin could be used as a prognostic marker in canine mammary neoplasia.