Resultado da pesquisa (32)
Termo utilizado na pesquisa Leishmaniasis
#31 - Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs, p.215-218
Abstract in English:
Lima V.M.F., Biazzono L., Silva A.C., Correa A.P.F.L. & Luvizotto M.C.R. 2005. Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs. Pesquisa Veterinária Brasileira 25(4):215-218. Departamento de Clínica, Cirurgia e Reprodução Animal, Faculdade de Medicina Veterinária, Universidade Estadual Paulista, Rua Clóvis Pestana 793, Araçatuba, SP 16050-680, Brazil. E-mail: vmflima@fmva.unesp.br
A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean optical density ± SD / 1.008 ± 0.437), while in the negative animals, the results by both systems were similar. These results suggest that the ELISA assay using protein A peroxidase conjugated could be useful to detect early infected animals in endemic areas, and thus help to control the spread of the infection.
Abstract in Portuguese:
Lima V.M.F., Biazzono L., Silva A.C., Correa A.P.F.L. & Luvizotto M.C.R. 2005. Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs. Pesquisa Veterinária Brasileira 25(4):215-218. Departamento de Clínica, Cirurgia e Reprodução Animal, Faculdade de Medicina Veterinária, Universidade Estadual Paulista, Rua Clóvis Pestana 793, Araçatuba, SP 16050-680, Brazil. E-mail: vmflima@fmva.unesp.br
A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean optical density ± SD / 1.008 ± 0.437), while in the negative animals, the results by both systems were similar. These results suggest that the ELISA assay using protein A peroxidase conjugated could be useful to detect early infected animals in endemic areas, and thus help to control the spread of the infection.
#32 - Epidemiological situation of visceral Leishmaniasis in dogs in Corumbá, Mato Grosso do Sul, Brazil
Abstract in English:
Recent reports on human cases with a clinical diagnosis of visceral Leishmaniasis in Corumbá, Mato Grosso do Sul state, where the agent had never been demonstrated parasitologically, caused a more thorough investigation of the local epidemiological situation of the dog population, one of the main reservoirs of the protozoa in question. Amastigotes were observed in parasitological examinations of skin and viscera in 7 out of 30 animals with clinical symptoms of kala-azar. The isolated parasites from infected dogs were identified as Leishmania donovani by biochemical methods. A serological survey was also carried out on 481 dogs from the urban area, by collecting blood on filter paper for indirect immunofluorescence and passive hemagglutination tests: The serological prevalence of the infection in the dog population studied was 8.7%, of which only 19% showed clinical signs of kala-azar. The remaining 81% was suggestive of being potential carriers without, however, ruling out the possibility of cross reactions with other animal protozoa. The canine infection. is dissiminated in practically all of the town.
Abstract in Portuguese:
Recentes relatos sobre casos humanos com diagnóstico clínico de leishmaniose visceral em Corumbá, Mato Grosso do Sul, onde o agente nunca havia sido demonstrado parasitologicamente, levou a uma melhor investigação epidemiológica local sobre a situação da população canina, um dos principais reservatórios do parasita. Formas amastigotas foram observadas através de exame parasitológico da pele e de vísceras de 7 entre 30 animais com sintomas clínicos de calazar. Os parasitas isolados de cães infectados foram identificados bioquímicamente como sendo Leishmania donovani. Inquérito sorológico foi também realizado em 481 cães da área urbana, através das reações de imunofluorescência indireta e hemaglutinação passiva, utilizando-se sangue coletado em papel de filtro. Sorologicamente, a prevalência da infecção na população canina estudada foi de 8,7%, dos quais, apenas 19% apresentavam sinais clínicos de calazar e o restante, 81 %, eram sugestivos de serem portadores em potencial, sem contudo, poder ser excluída a possibilidade de reações cruzadas com outros protozoários. A infecção canina encontra-se praticamente disseminada por toda a cidade.
