PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Barbosa J.D., Albernaz T.T., Riet-Correa G, Cerqueira V.D., Soares S.O., Campos K.F., Oliveira C.M.C. & Duarte M.D. 2009. [Angular cheilitis in horses associated with ingestion of Panicum maximum.] Queilite angular traumática em eqüinos associada à ingestão de Panicum maximum. Pesquisa Veterinária Brasileira 29(5):428-430. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition with enlargement of the oral cleft in horses was studied. The enlargement of varied extension was uni or bilateral. The cheek mucosa of the labial commissure showed slight erosions. During chewing there was loss of small amounts of grass and saliva through the oral cleft. The affected horses were in good nutritional condition. Histopathological studies of tissues obtained by biopsia, revealed a superficial epidermitis. The pastures consisted of Panicum maximum grass (varieties Tanzânia, Mombaça, Tobiatã and Colonião) which was mature, tall, lignified, with leaves of cutting edges. Based on epidemiological, clinical and histopathological data, it was concluded that the lesions were caused by the hard grass, favored by the way horses pull the tall grass and chew it.

• Cheilitis Panicum maximum diseases of horses

Abstract in Portuguese:

ABSTRACT.- Barbosa J.D., Albernaz T.T., Riet-Correa G, Cerqueira V.D., Soares S.O., Campos K.F., Oliveira C.M.C. & Duarte M.D. 2009. [Angular cheilitis in horses associated with ingestion of Panicum maximum.] Queilite angular traumática em eqüinos associada à ingestão de Panicum maximum. Pesquisa Veterinária Brasileira 29(5):428-430. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition with enlargement of the oral cleft in horses was studied. The enlargement of varied extension was uni or bilateral. The cheek mucosa of the labial commissure showed slight erosions. During chewing there was loss of small amounts of grass and saliva through the oral cleft. The affected horses were in good nutritional condition. Histopathological studies of tissues obtained by biopsia, revealed a superficial epidermitis. The pastures consisted of Panicum maximum grass (varieties Tanzânia, Mombaça, Tobiatã and Colonião) which was mature, tall, lignified, with leaves of cutting edges. Based on epidemiological, clinical and histopathological data, it was concluded that the lesions were caused by the hard grass, favored by the way horses pull the tall grass and chew it.

Abstract in English:

ABSTRACT.- Grecco F.B., Schild A.L., Soares M.P., Raffi M.B., Sallis E.S.V. & Damé M.C. 2009. [Organophosphate poisoning in buffaloes (Bubalus bubalis) in southern Brazil.] Intoxicação por organofosforados em búfalos (Bubalus bubalis) no Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(3):211-214. Laboratório Regional de Diagnóstico, Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS 96010-900, Brazil. E-mail: alschild@terra.com.br This paper describes organophosphate (ORF) poisoning in a herd of water buffaloes (Bubalus bubalis) in southern Brazil, which were treated with a single dose of 12mg/kg body weight of Expertan® pour-on (chlorpyriphos) to control ectoparasites. Clinical signs, observed 7-45 days after exposure, were diarrhea, hypersalivation, ataxia, muscular tremors, weakness of pelvic limbs, paresis and flaccid paralysis and lateral recumbence. Out of 267 buffaloes 61 died. Necropsy of three animals that died 24-72 hours after onset of clinical signs, revealed congestion and serosal hemorrhages scattered along the bowel, emphysema and edema of the lungs. No significant histopathological changes were found. Residues of chlorpyriphos were detected in liver, kidneys and nervous system of the one necropsied buffalo. Despite the absence of histological lesions in the central and peripheral nervous system, the epidemiological, clinical, gross and toxicological findings suggest delayed neurotoxicity induced by organophosphates.

• Diseases of water buffaloes poisoning organophosphate toxicosis

Abstract in Portuguese:

ABSTRACT.- Grecco F.B., Schild A.L., Soares M.P., Raffi M.B., Sallis E.S.V. & Damé M.C. 2009. [Organophosphate poisoning in buffaloes (Bubalus bubalis) in southern Brazil.] Intoxicação por organofosforados em búfalos (Bubalus bubalis) no Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(3):211-214. Laboratório Regional de Diagnóstico, Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS 96010-900, Brazil. E-mail: alschild@terra.com.br This paper describes organophosphate (ORF) poisoning in a herd of water buffaloes (Bubalus bubalis) in southern Brazil, which were treated with a single dose of 12mg/kg body weight of Expertan® pour-on (chlorpyriphos) to control ectoparasites. Clinical signs, observed 7-45 days after exposure, were diarrhea, hypersalivation, ataxia, muscular tremors, weakness of pelvic limbs, paresis and flaccid paralysis and lateral recumbence. Out of 267 buffaloes 61 died. Necropsy of three animals that died 24-72 hours after onset of clinical signs, revealed congestion and serosal hemorrhages scattered along the bowel, emphysema and edema of the lungs. No significant histopathological changes were found. Residues of chlorpyriphos were detected in liver, kidneys and nervous system of the one necropsied buffalo. Despite the absence of histological lesions in the central and peripheral nervous system, the epidemiological, clinical, gross and toxicological findings suggest delayed neurotoxicity induced by organophosphates.

Abstract in English:

ABSTRACT.- Pierezan F., Rissi D.R., Rech R.R., Fighera R.A. Brum J.S. & Barros C.S.L. 2009. [Necropsy findings related to the cause of death in 335 horses: 1968-2007.] Achados de necropsia relacionados com a morte de 335 eqüinos: 1968-2007. Pesquisa Veterinária Brasileira 29(3):275-280. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: claudioslbarros@uol.com.br The necropsy reports of 335 horses necropsied at the LPV-UFSM between 1968-2007 were reviewed in order to determine the necropsy findings related with cause of death or reason for euthanasia. The distribution of these findings by organ system were as follows: digestive (79/335 [23.6%]), striated muscle and skeleton (47/335 [14.0%]), nervous (37/335 [11.0%]), respiratory (35/335 [10.4%]), integument (31/335 [9.3%]), hematopoietic (24/335 [7.2%]), cardiovascular (13/335 [3.9%]), reproductive (12/335 [3.5%]), urinary (7/335 [2.1%]), and endocrine (3/335 [0.9%]). The cause of death was not possible to be determined in 47 (14.0%) necropsied horses. Displacements of the intestines (17/79 [21.5%]) were the main findings in digestive system, followed by obstruction and impactation (14/79 [17.7%]). Torsion were the type of displacement more frequently observed in the intestines (14/17 [82.4%]). Among those the more prevalent affected the small intestine (7/14 [50%]). Most horses dying from fractured bones were 1-5-year-old. The most prevalent diseases in the nervous system were leukoencephalo-malacia and trypanosomiasis, whereas respiratory depression due to anesthesia was the leading cause of death related to the respiratory system. Equine infectious anemia was the most diagnosed infectious disease and the main reason leading to euthanasia.

• Diseases of horses causes of death epidemiology pathology

Abstract in Portuguese:

ABSTRACT.- Pierezan F., Rissi D.R., Rech R.R., Fighera R.A. Brum J.S. & Barros C.S.L. 2009. [Necropsy findings related to the cause of death in 335 horses: 1968-2007.] Achados de necropsia relacionados com a morte de 335 eqüinos: 1968-2007. Pesquisa Veterinária Brasileira 29(3):275-280. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: claudioslbarros@uol.com.br The necropsy reports of 335 horses necropsied at the LPV-UFSM between 1968-2007 were reviewed in order to determine the necropsy findings related with cause of death or reason for euthanasia. The distribution of these findings by organ system were as follows: digestive (79/335 [23.6%]), striated muscle and skeleton (47/335 [14.0%]), nervous (37/335 [11.0%]), respiratory (35/335 [10.4%]), integument (31/335 [9.3%]), hematopoietic (24/335 [7.2%]), cardiovascular (13/335 [3.9%]), reproductive (12/335 [3.5%]), urinary (7/335 [2.1%]), and endocrine (3/335 [0.9%]). The cause of death was not possible to be determined in 47 (14.0%) necropsied horses. Displacements of the intestines (17/79 [21.5%]) were the main findings in digestive system, followed by obstruction and impactation (14/79 [17.7%]). Torsion were the type of displacement more frequently observed in the intestines (14/17 [82.4%]). Among those the more prevalent affected the small intestine (7/14 [50%]). Most horses dying from fractured bones were 1-5-year-old. The most prevalent diseases in the nervous system were leukoencephalo-malacia and trypanosomiasis, whereas respiratory depression due to anesthesia was the leading cause of death related to the respiratory system. Equine infectious anemia was the most diagnosed infectious disease and the main reason leading to euthanasia.

Abstract in English:

Abstract.- Sonne L., Oliveira E.C., Pescador C.A., Santos A.S., Pavarini S. P., Carissimi A.S. & Driemeier D. 2009. [Pathologic and immunohistochemistry findings in dogs naturally infected by canine distemper virus.] Achados patológicos e imuno-histoquímicos em cães infectados naturalmente pelo vírus da cinomose canina. Pesquisa Veterinária Brasileira 29(2):143-149. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br Canine distemper is a viral disease that affects mainly respiratory, gastrointestinal and nervous system. The present study analyzes pathologic and immunohistochemical findings in 54 dogs with canine distemper of a total of 760 canine necropsies performed from July 2006 to October 2007. The gross lesions were characterized by mucopurulent oculonasal discharge, hyperkeratosis of footpads, red and not collapsed lungs, thymic atrophy, watery intestinal content, hyperemia and enlarged Peyer’s patches. The histological findings were characterized by interstitial pneumonia, lymphoid depletion, white matter demyelization, perivascular cuffs and intracytoplasmatic and intranuclear inclusion bodies located in epithelial cells of gastric mucosa, urinary bladder, bronchial, renal pelvis, footpads, eyelid, skin of the ear, tonsil, central nervous system and mononuclear cells in lymph nodes, spleen, and tonsils. Viral antigen was detected by an immunohistochemical procedure using a mouse monoclonal anti-canine distemper antibody. The footpads were the more constantly (67.4% of the cases) immunolabeled tissue, followed by stomach with 62.7%. Immunohistochemistry was demonstrated to be a useful tool for the study of viral antigen distribution in distemper affected dogs as well as it indicated which is the best tissue to be examined in order to confirm a suspected case of canine.

• Canine diseases distemper immunohistochemistry

Abstract in Portuguese:

Abstract.- Sonne L., Oliveira E.C., Pescador C.A., Santos A.S., Pavarini S. P., Carissimi A.S. & Driemeier D. 2009. [Pathologic and immunohistochemistry findings in dogs naturally infected by canine distemper virus.] Achados patológicos e imuno-histoquímicos em cães infectados naturalmente pelo vírus da cinomose canina. Pesquisa Veterinária Brasileira 29(2):143-149. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br Canine distemper is a viral disease that affects mainly respiratory, gastrointestinal and nervous system. The present study analyzes pathologic and immunohistochemical findings in 54 dogs with canine distemper of a total of 760 canine necropsies performed from July 2006 to October 2007. The gross lesions were characterized by mucopurulent oculonasal discharge, hyperkeratosis of footpads, red and not collapsed lungs, thymic atrophy, watery intestinal content, hyperemia and enlarged Peyer’s patches. The histological findings were characterized by interstitial pneumonia, lymphoid depletion, white matter demyelization, perivascular cuffs and intracytoplasmatic and intranuclear inclusion bodies located in epithelial cells of gastric mucosa, urinary bladder, bronchial, renal pelvis, footpads, eyelid, skin of the ear, tonsil, central nervous system and mononuclear cells in lymph nodes, spleen, and tonsils. Viral antigen was detected by an immunohistochemical procedure using a mouse monoclonal anti-canine distemper antibody. The footpads were the more constantly (67.4% of the cases) immunolabeled tissue, followed by stomach with 62.7%. Immunohistochemistry was demonstrated to be a useful tool for the study of viral antigen distribution in distemper affected dogs as well as it indicated which is the best tissue to be examined in order to confirm a suspected case of canine.

Abstract in English:

ABSTRACT.- Souza A.I., Oliveira T.M.F.S., Machado R.Z. & Camacho A.A. 2009. [Seroprevalence of infection by Trypanosoma cruzi in dogs in a rural area of Mato Grosso do Sul, Brazil.] Soroprevalência da infecção por Trypanosoma cruzi em cães de uma área rural do Estado de Mato Grosso do Sul. Pesquisa Veterinária Brasileira 29(2):150-152. Curso de Medicina Veterinária, Universidade para o Desenvolvimento do Estado e da Região do Pantanal, Rua Alexandre Herculano 1400, Jardim Veraneio, Campo Grande, MS 79037-280, Brazil. E-mail: aldaizabel@hotmail.com Chagas disease is an anthropozoonosis caused by Trypanosoma cruzi and dogs are considered to be one of the main reservoirs of the disease in the South America. This study evaluates the occurrence of the infection caused by T. cruzi in dogs from a rural area of Mato Grosso do Sul, Brazil. Indirect immunofluorescence test (IFI) and enzyme-linked immunosorbent assay (ELISA) was used in 75 dogs living in this area. The antibodies were detected in 45.3% (n=34) and 24.0% (n=18) with IFI and ELISA, respectively. The actual prevalence of the infection was confirmed as 22.7% (n=17) by the criterion of positivity in both tests. The results confirm T. cruzi infection in dogs in the region.

• Diseases of dogs Trypanosoma cruzi seroprevalence Mato Grosso do Sul

Abstract in Portuguese:

ABSTRACT.- Souza A.I., Oliveira T.M.F.S., Machado R.Z. & Camacho A.A. 2009. [Seroprevalence of infection by Trypanosoma cruzi in dogs in a rural area of Mato Grosso do Sul, Brazil.] Soroprevalência da infecção por Trypanosoma cruzi em cães de uma área rural do Estado de Mato Grosso do Sul. Pesquisa Veterinária Brasileira 29(2):150-152. Curso de Medicina Veterinária, Universidade para o Desenvolvimento do Estado e da Região do Pantanal, Rua Alexandre Herculano 1400, Jardim Veraneio, Campo Grande, MS 79037-280, Brazil. E-mail: aldaizabel@hotmail.com Chagas disease is an anthropozoonosis caused by Trypanosoma cruzi and dogs are considered to be one of the main reservoirs of the disease in the South America. This study evaluates the occurrence of the infection caused by T. cruzi in dogs from a rural area of Mato Grosso do Sul, Brazil. Indirect immunofluorescence test (IFI) and enzyme-linked immunosorbent assay (ELISA) was used in 75 dogs living in this area. The antibodies were detected in 45.3% (n=34) and 24.0% (n=18) with IFI and ELISA, respectively. The actual prevalence of the infection was confirmed as 22.7% (n=17) by the criterion of positivity in both tests. The results confirm T. cruzi infection in dogs in the region.

Abstract in English:

ABSTRACT.- Pereira I.A., Soares L.C., Coelho S.M.O., Pribul B.R. & Souza M.M.S. 2009. [Susceptibility to azithromycin of bacteria isolated from infectious processes in dogs and cats.] Suscetibilidade à azitromicina de isolados bacterianos de processos infecciosos em cães e gatos. Pesquisa Veterinária Brasileira 29(2):153-156. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. E-mail: miliane@ufrrj.br The susceptibility pattern to azithromycin of bacterial pathogens from various infectious sites, and the in vitro activity and minimum inhibitory concentration (MIC) of azithromycin were studied. Tests such as disc diffusion and broth microdilution detected respectively 48.6% and 55% of resistant Staphylococcus spp., and 55.3% and 72.7% resistant gram-negative rods. MIC50 for S. aureus was 4.0mg/mL, that for S. intermedius was 1.0mg/mL, for coagulase-negative Staphylococcus e”512mg/mL, and for gram-negative rods 256mg/mL. Fifteen percent (9/60) of oxacilin-resistant, multidrug-resistant and mecA-positive Staphylococcus spp. isolates were also azithromycin resistant. The dissemination of multidrug resistant bacteria points out to the need of antimicrobial evaluation activity in order to select the best indicated drug and thus minimizing therapeutic failures in veterinary practice.

• Diseases of dogs and cats bacterial diseases bacterial antibiotic resistance azithromycin

Abstract in Portuguese:

ABSTRACT.- Pereira I.A., Soares L.C., Coelho S.M.O., Pribul B.R. & Souza M.M.S. 2009. [Susceptibility to azithromycin of bacteria isolated from infectious processes in dogs and cats.] Suscetibilidade à azitromicina de isolados bacterianos de processos infecciosos em cães e gatos. Pesquisa Veterinária Brasileira 29(2):153-156. Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ 23890-000, Brazil. E-mail: miliane@ufrrj.br The susceptibility pattern to azithromycin of bacterial pathogens from various infectious sites, and the in vitro activity and minimum inhibitory concentration (MIC) of azithromycin were studied. Tests such as disc diffusion and broth microdilution detected respectively 48.6% and 55% of resistant Staphylococcus spp., and 55.3% and 72.7% resistant gram-negative rods. MIC50 for S. aureus was 4.0mg/mL, that for S. intermedius was 1.0mg/mL, for coagulase-negative Staphylococcus e”512mg/mL, and for gram-negative rods 256mg/mL. Fifteen percent (9/60) of oxacilin-resistant, multidrug-resistant and mecA-positive Staphylococcus spp. isolates were also azithromycin resistant. The dissemination of multidrug resistant bacteria points out to the need of antimicrobial evaluation activity in order to select the best indicated drug and thus minimizing therapeutic failures in veterinary practice.

Abstract in English:

ABSTRACT.- Souza T.M., Fighera R.A., Schmidt C., Réquia A.H., Brum J.S., Martins T.B. & Barros C.S.L. 2009. [Prevalence of non-tumorous canine dermatopathies in dogs from the municipality of Santa Maria, Rio Grande do Sul, Brazil (2005-2008).] Prevalência das dermatopatias não-tumorais em cães do município de Santa Maria, Rio Grande do Sul (2005-2008). Pesquisa Veterinária Brasileira 29(2):157-162. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: teitsouza@yahoo.com.br The current study was aimed at determine the prevalence of non-tumorous canine dermatopathies affecting dogs from the municipality of Santa Maria, Rio Grande do Sul, Brazil. From March 2005 to June de 2008, the authors followed-up canine dermatological cases from two sources: those referred to the Dermatology Sector of the Veterinary Teaching Hospital at the Universidade Federal de Santa Maria and those from a private practice. During this period 480 dogs with dermatological problems were examined; in 393 (81.9%) it was possible to establish a definitive diagnosis and in 87 (18.1%) the diagnosis was inconclusive. Four hundred and twenty four primary diagnosis and 78 secondary diagnosis were performed in the 393 dogs with conclusive diagnosis, totaling 502 diagnosis. The distribution of the diagnosis according to the categories of diagnosed dermatopathies was as follows: Allergic (190/502 [37.8%]), bacterial (103/502 [20.5%]), parasitic (97/502 [19.3%]), environmental (28/502 [5,6%]), mycotic (20/502 [4.0%]), endocrine (13/502 [2.6%]), keratinization disturbances (11/502 [2.2%]), psychogenic (9/502 [1.8%]), acquired alopecias (6/502 [1.2%]), autoimmune (6/502 [1.2%]), inherited (6/502 [1.2%]), pigmentary disturbances (1/502 [0.2%], nutritional (1/502 [0.2%]), and sundry conditions (11/502 [2.2%]). In general, the ten most frequently diagnosed non-tumorous dermatopathies in decreasing order of frequency were: Atopy, flea bite allergic dermatitis, bacterial folliculitis, demodectic mange, deep bacterial folliculitis/furunculosis, sarcoptic mange, myiasis, food allergy, traumatic pyoderma, and Malassezia dermatitis. These 10 conditions together made up approximately for three quarters of all canine skin diseases diagnosed in the current study.

• Diseases of dogs skin diseases dermatology dermatopathology

Abstract in Portuguese:

ABSTRACT.- Souza T.M., Fighera R.A., Schmidt C., Réquia A.H., Brum J.S., Martins T.B. & Barros C.S.L. 2009. [Prevalence of non-tumorous canine dermatopathies in dogs from the municipality of Santa Maria, Rio Grande do Sul, Brazil (2005-2008).] Prevalência das dermatopatias não-tumorais em cães do município de Santa Maria, Rio Grande do Sul (2005-2008). Pesquisa Veterinária Brasileira 29(2):157-162. Departamento de Patologia, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil. E-mail: teitsouza@yahoo.com.br The current study was aimed at determine the prevalence of non-tumorous canine dermatopathies affecting dogs from the municipality of Santa Maria, Rio Grande do Sul, Brazil. From March 2005 to June de 2008, the authors followed-up canine dermatological cases from two sources: those referred to the Dermatology Sector of the Veterinary Teaching Hospital at the Universidade Federal de Santa Maria and those from a private practice. During this period 480 dogs with dermatological problems were examined; in 393 (81.9%) it was possible to establish a definitive diagnosis and in 87 (18.1%) the diagnosis was inconclusive. Four hundred and twenty four primary diagnosis and 78 secondary diagnosis were performed in the 393 dogs with conclusive diagnosis, totaling 502 diagnosis. The distribution of the diagnosis according to the categories of diagnosed dermatopathies was as follows: Allergic (190/502 [37.8%]), bacterial (103/502 [20.5%]), parasitic (97/502 [19.3%]), environmental (28/502 [5,6%]), mycotic (20/502 [4.0%]), endocrine (13/502 [2.6%]), keratinization disturbances (11/502 [2.2%]), psychogenic (9/502 [1.8%]), acquired alopecias (6/502 [1.2%]), autoimmune (6/502 [1.2%]), inherited (6/502 [1.2%]), pigmentary disturbances (1/502 [0.2%], nutritional (1/502 [0.2%]), and sundry conditions (11/502 [2.2%]). In general, the ten most frequently diagnosed non-tumorous dermatopathies in decreasing order of frequency were: Atopy, flea bite allergic dermatitis, bacterial folliculitis, demodectic mange, deep bacterial folliculitis/furunculosis, sarcoptic mange, myiasis, food allergy, traumatic pyoderma, and Malassezia dermatitis. These 10 conditions together made up approximately for three quarters of all canine skin diseases diagnosed in the current study.

Abstract in English:

ABSTRACT.- Iamaguti P., Iamaguti L.S. & Sartor R. 2009. Acetabular deepening in the treatment of severe canine hip dysplasia. Pesquisa Veterinária Brasileira 29(2):163-166. Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-000, Brazil. E-mail: raquelsartor@yahoo.com.br A technique to restore acetabular anatomy by deepening the acetabular cavity and reconstructing the femoral head ligament and the joint capsule was tested on nine large breed dogs with severe hip dysplasia and acute subdislocation or dislocation. The technique consisted of two phases. First, all dogs were submitted to bilateral pectinotomy. In a second surgical intervention on the same dogs the acetabulum was approached and deepened, and the femoral head ligament and the joint capsule were reconstructed. In general, within 30 days of the surgery dogs could stand on the operated member to walk. Except for two dogs, all the others recovered pelvic member locomotive ability within 60-90 days after surgery. It is concluded that acetabuloplasty is a good alternative for treatment of severe canine hip dysplasia.

• Diseases of dogs surgery hip dysplasia acetabuloplasty

Abstract in Portuguese:

ABSTRACT.- Iamaguti P., Iamaguti L.S. & Sartor R. 2009. Acetabular deepening in the treatment of severe canine hip dysplasia. Pesquisa Veterinária Brasileira 29(2):163-166. Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-000, Brazil. E-mail: raquelsartor@yahoo.com.br A technique to restore acetabular anatomy by deepening the acetabular cavity and reconstructing the femoral head ligament and the joint capsule was tested on nine large breed dogs with severe hip dysplasia and acute subdislocation or dislocation. The technique consisted of two phases. First, all dogs were submitted to bilateral pectinotomy. In a second surgical intervention on the same dogs the acetabulum was approached and deepened, and the femoral head ligament and the joint capsule were reconstructed. In general, within 30 days of the surgery dogs could stand on the operated member to walk. Except for two dogs, all the others recovered pelvic member locomotive ability within 60-90 days after surgery. It is concluded that acetabuloplasty is a good alternative for treatment of severe canine hip dysplasia.

Abstract in English:

ABSTRACT.- Coutinho A.S., Oliveira Filho J.P., Silva D.P.G.S., Oliveira A.P., Marcondes J.S., Chiacchio S.B., Paes A.C., Amorim R.M. & Gonçalves R.C. 2009. [Experimental pneumonic mannheimiosis in calves: Nasal and nasopharingeal swabs for diagnostic.] Mannheimiose pulmonar experimental em bezerros: swab nasal e nasofaringeano como auxílio diagnóstico. Pesquisa Veterinária Brasileira 29(1):83-88. Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618000, Brazil. E-mail: zep.filho@hotmail.com An experimental model of bovine pneumonic mannheimiosis (BPM) was used to evaluate the nasal and nasopharynx bacterial species of calves during the course of the disease and for checking the diagnostic efficiency of nasal swab (NS) and nasopharingeal swab (NPS) microbiological exams. A total of 28 calves were randomized into four experimental groups (G1-G4). NS and NPS were obtained 7 days before and 12 (G1), 24 (G2), 48 (G3) e 72 (G4) hours after intrabronchial inoculation of Mannheimia haemolytica. After the induction of BPM, M. haemolytica biotype A was the predominant isolated bacterium in NS and NPS in all evaluated sampling times, except for one NS (harvested 24 hours). There were no significant statistical differences for the rates of Pasteurella multocida isolation in NS and NPS, harvested before and after the induction of BPM. However, this bacterium was isolated more frequently after the induction of BPM, mainly in NPS. Therefore, the microbiological NS and NPS exams were important auxiliary tests for diagnosing BPM.

• Bacterial flora upper airway respiratory diseases bovine Mannheimia haemolytica

Abstract in Portuguese:

ABSTRACT.- Coutinho A.S., Oliveira Filho J.P., Silva D.P.G.S., Oliveira A.P., Marcondes J.S., Chiacchio S.B., Paes A.C., Amorim R.M. & Gonçalves R.C. 2009. [Experimental pneumonic mannheimiosis in calves: Nasal and nasopharingeal swabs for diagnostic.] Mannheimiose pulmonar experimental em bezerros: swab nasal e nasofaringeano como auxílio diagnóstico. Pesquisa Veterinária Brasileira 29(1):83-88. Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618000, Brazil. E-mail: zep.filho@hotmail.com An experimental model of bovine pneumonic mannheimiosis (BPM) was used to evaluate the nasal and nasopharynx bacterial species of calves during the course of the disease and for checking the diagnostic efficiency of nasal swab (NS) and nasopharingeal swab (NPS) microbiological exams. A total of 28 calves were randomized into four experimental groups (G1-G4). NS and NPS were obtained 7 days before and 12 (G1), 24 (G2), 48 (G3) e 72 (G4) hours after intrabronchial inoculation of Mannheimia haemolytica. After the induction of BPM, M. haemolytica biotype A was the predominant isolated bacterium in NS and NPS in all evaluated sampling times, except for one NS (harvested 24 hours). There were no significant statistical differences for the rates of Pasteurella multocida isolation in NS and NPS, harvested before and after the induction of BPM. However, this bacterium was isolated more frequently after the induction of BPM, mainly in NPS. Therefore, the microbiological NS and NPS exams were important auxiliary tests for diagnosing BPM.

Abstract in English:

ABSTRACT.- Gabriel A.L., Kommers G.D., Trost M.E., Barros C.S.L., Pereira D.B., Schwendler S.E. & Santurio J.M. 2008. [Outbreak of cutaneous pythiosis in cattle.] Surto de pitiose cutânea em bovinos. Pesquisa Veterinária Brasileira 28(12):583-587. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: glaukommers@yahoo.com Seventy-six young mixed breed cattle of both sexes, presented multifocal ulcerated nodular cutaneous lesions localized in the medial and lateral aspects of fore and hindlimbs, ventral neck, sternum, and tail. The disease occurred during summer and lesions were observed on areas of the body which were in contact with water of irrigation channels for long periods. Histologically, there were multiple granulomas and pyogranulomas with few negative profiles of hyphae, which were better visualized throughout Grocott methenamine silver stain. Definitive etiologic diagnosis was based on immuno-histochemistry with anti-Pythium insidiosum polyclonal antibody. Additionally, an indirect ELISA test was performed. Bovine cutaneous pythiosis outbreaks are uncommon and, particularly as occurred in the cattle of this report, all affected animals had spontaneous healing within two to three weeks.

• Pythiosis Pythium insidiosum diseases of cattle immunohistochemistry ELISA test pathology

Abstract in Portuguese:

ABSTRACT.- Gabriel A.L., Kommers G.D., Trost M.E., Barros C.S.L., Pereira D.B., Schwendler S.E. & Santurio J.M. 2008. [Outbreak of cutaneous pythiosis in cattle.] Surto de pitiose cutânea em bovinos. Pesquisa Veterinária Brasileira 28(12):583-587. Departamento de Patologia, Universidade Federal de Santa Maria, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: glaukommers@yahoo.com Seventy-six young mixed breed cattle of both sexes, presented multifocal ulcerated nodular cutaneous lesions localized in the medial and lateral aspects of fore and hindlimbs, ventral neck, sternum, and tail. The disease occurred during summer and lesions were observed on areas of the body which were in contact with water of irrigation channels for long periods. Histologically, there were multiple granulomas and pyogranulomas with few negative profiles of hyphae, which were better visualized throughout Grocott methenamine silver stain. Definitive etiologic diagnosis was based on immuno-histochemistry with anti-Pythium insidiosum polyclonal antibody. Additionally, an indirect ELISA test was performed. Bovine cutaneous pythiosis outbreaks are uncommon and, particularly as occurred in the cattle of this report, all affected animals had spontaneous healing within two to three weeks.