PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Pagliosa G.M., Alves G.E.S., Faleiros R.R., Matos J.R.T., Torres M.B., Mendes H.M.F. & Costa R.S. 2009. [Use of glutamine to treatment of ischemia and reperfusion lesions in equine jejune.] Utilização da glutamina no tratamento de lesões de isquemia e reperfusão no jejuno em eqüinos. Pesquisa Veterinária Brasileira 29(3):246-252. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Ave-nida Antônio Carlos 6627, Caixa Postal 567, Belo Horizonte, MG 30123-970, Brazil. E-mail: geanepagliosa@gmail.com Under general anesthesia and arterial pressure control, twelve horses were submitted simultaneously to intraluminal distension and venous ischemia of jejune for 2 hours, followed by 12 hours of reperfusion. Six horses were treated with intravenous 2% glutamine (50mg/kg), and six others were treated with the same volume of saline solution 1h after the beginning of ischemia and 6 hours after reperfusion. Intestinal samples were collected before and after 2 hours of ischemia and after 2 and 12 hours of reperfusion, and were submitted to hystopatological analyses, ultrastructural examination, measurement of area and number of villi per mm2, and to myeloperoxidases activity. No statistical differences between the groups were found. It was concluded that glutamine did not attenuate the ischemia and reperfusion lesions under the methodology used in this study.

• Intestine ischemia and reperfusion colic glutamine equine

Abstract in Portuguese:

ABSTRACT.- Pagliosa G.M., Alves G.E.S., Faleiros R.R., Matos J.R.T., Torres M.B., Mendes H.M.F. & Costa R.S. 2009. [Use of glutamine to treatment of ischemia and reperfusion lesions in equine jejune.] Utilização da glutamina no tratamento de lesões de isquemia e reperfusão no jejuno em eqüinos. Pesquisa Veterinária Brasileira 29(3):246-252. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Ave-nida Antônio Carlos 6627, Caixa Postal 567, Belo Horizonte, MG 30123-970, Brazil. E-mail: geanepagliosa@gmail.com Under general anesthesia and arterial pressure control, twelve horses were submitted simultaneously to intraluminal distension and venous ischemia of jejune for 2 hours, followed by 12 hours of reperfusion. Six horses were treated with intravenous 2% glutamine (50mg/kg), and six others were treated with the same volume of saline solution 1h after the beginning of ischemia and 6 hours after reperfusion. Intestinal samples were collected before and after 2 hours of ischemia and after 2 and 12 hours of reperfusion, and were submitted to hystopatological analyses, ultrastructural examination, measurement of area and number of villi per mm2, and to myeloperoxidases activity. No statistical differences between the groups were found. It was concluded that glutamine did not attenuate the ischemia and reperfusion lesions under the methodology used in this study.

Abstract in English:

ABSTRACT.- Botteon P.T.L., Ferraz V.P., Glória M.B.A., Melo M.M., Paes Leme F.O., Faleiros R.R. & Alves G.E.S. 2008. [Plasmatic concentrations of tryptamine, tyramine end phenylethylamine in horses under the effect of carbohydrate overload and non-steroid antinflammatory compounds.] Concentrações plasmáticas de triptamina, tiramina e feniletilamina em eqüinos sob efeitos de sobrecarga de carboidratos e antiinflamatórios não esteroidais. Pesquisa Veterinária Brasileira 28(6):299-302. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, 30123-970, Brazil. E-mail: paulobotteon@gmail.com The concentrations of the bioactives amines tryptamine (TRP), tyramine (TYR) and phenylethylamine (PEA) were determined by gas chromatography in plasma samples of 20 horses submitted to carbohydrate overload. Thirty hours after the overload, the horses were randomly distributed in four groups (n=5) and were submitted to four IV treatments every 12 hours: 10ml of saline (GC), ketoprofen 2.2mg/kg (GK), phenylbutazone 4.4mg/kg (GF), and flunixin meglumine 1.1mg/kg (GFM). Blood samples were collected at various times after the overload (0-72 h). Plasma TYR and PEA concentrations ranged from 0.18 to 164.2mg/L, and differed significantly with time (p<0.01), but did not differ in the treatments. Plasma concentrations of TRP differed between times and treatments. The GC was significantly major than other treatments at 48h and 60h after the overload, and the plasma concentration of TRP in groups GK and GFM was significantly lower than in groups GF and GC at 72 h (p=0.0012). We concluded that the anti-inflammatory drugs evaluated do not interfere in the plasma concentration of TYP and PEA. For TRP, ketoprofen and flunixin meglumine was effective to reduce de plasmatic concentration of this amine.

• Amines antinflammatory compounds laminitis horse

Abstract in Portuguese:

ABSTRACT.- Botteon P.T.L., Ferraz V.P., Glória M.B.A., Melo M.M., Paes Leme F.O., Faleiros R.R. & Alves G.E.S. 2008. [Plasmatic concentrations of tryptamine, tyramine end phenylethylamine in horses under the effect of carbohydrate overload and non-steroid antinflammatory compounds.] Concentrações plasmáticas de triptamina, tiramina e feniletilamina em eqüinos sob efeitos de sobrecarga de carboidratos e antiinflamatórios não esteroidais. Pesquisa Veterinária Brasileira 28(6):299-302. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, Belo Horizonte, MG, 30123-970, Brazil. E-mail: paulobotteon@gmail.com The concentrations of the bioactives amines tryptamine (TRP), tyramine (TYR) and phenylethylamine (PEA) were determined by gas chromatography in plasma samples of 20 horses submitted to carbohydrate overload. Thirty hours after the overload, the horses were randomly distributed in four groups (n=5) and were submitted to four IV treatments every 12 hours: 10ml of saline (GC), ketoprofen 2.2mg/kg (GK), phenylbutazone 4.4mg/kg (GF), and flunixin meglumine 1.1mg/kg (GFM). Blood samples were collected at various times after the overload (0-72 h). Plasma TYR and PEA concentrations ranged from 0.18 to 164.2mg/L, and differed significantly with time (p<0.01), but did not differ in the treatments. Plasma concentrations of TRP differed between times and treatments. The GC was significantly major than other treatments at 48h and 60h after the overload, and the plasma concentration of TRP in groups GK and GFM was significantly lower than in groups GF and GC at 72 h (p=0.0012). We concluded that the anti-inflammatory drugs evaluated do not interfere in the plasma concentration of TYP and PEA. For TRP, ketoprofen and flunixin meglumine was effective to reduce de plasmatic concentration of this amine.

Abstract in English:

ABSTRACT.- Faleiros R.R., Macoris D.G., Alves G.E.S., Saquetti C.H.C. & Alessi A.C. 2007. [Histomorphometric and ultrastructural evaluation of the mucosa of the equine small colon subjected to distention.] Avaliação histomorfométrica e ultra-estrutural da mucosa do cólon menor eqüino submetido à distensão. Pesquisa Veterinária Brasileira 27(9):383-387. Departamento de Clínica e Cirurgia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Campus de Jaboticabal, Rodovia Carlos Tonanni Km 5, Jaboticabal, SP 14870-000, Brazil. E-mail: faleiros@ufmg.br Recently it has been shown that experimental distention of the small colon of horses promotes reduction of microvascular circulation and inflammation of the seromuscular layer associated with neutrophil accumulation in the lungs. However this model was not sufficient to induce evident histophatological changes in the mucosal layer. The aim of this study was to evaluate the mucosa subjected to that model of small colon distention by histomorphometry and scan electronic microscopy (SEM). Sixteen horses were used. In the distended group (DG), nine of them were subjected to distention of the small colon by a surgically implanted intraluminal balloon that was inflated with a pressure of 40mm Hg during 4 hours. In the sham-operated group (SG), the balloon was implanted but not inflated. Full-thickness intestinal samples were collected before and after obstruction and after 1.5 and 12 hours of decom-pression. By SEM, it was observed that the mucosa turned flat and smooth after distention and returned to the wrinkled original appearance after decompression. Twelve hours after decompression the mucosa had a more irregular appearance with points of fragmentation. There was a reduction in mucosa thickness after distention, returning to basal values after decompression. Instead of the fact that there were changes in appearance and thickness, it was concluded that the mucosa could borne up the compression caused by distention returning to the original characteristics without major lesions.

• Small colon intestinal distention colic horse

Abstract in Portuguese:

ABSTRACT.- Faleiros R.R., Macoris D.G., Alves G.E.S., Saquetti C.H.C. & Alessi A.C. 2007. [Histomorphometric and ultrastructural evaluation of the mucosa of the equine small colon subjected to distention.] Avaliação histomorfométrica e ultra-estrutural da mucosa do cólon menor eqüino submetido à distensão. Pesquisa Veterinária Brasileira 27(9):383-387. Departamento de Clínica e Cirurgia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Campus de Jaboticabal, Rodovia Carlos Tonanni Km 5, Jaboticabal, SP 14870-000, Brazil. E-mail: faleiros@ufmg.br Recently it has been shown that experimental distention of the small colon of horses promotes reduction of microvascular circulation and inflammation of the seromuscular layer associated with neutrophil accumulation in the lungs. However this model was not sufficient to induce evident histophatological changes in the mucosal layer. The aim of this study was to evaluate the mucosa subjected to that model of small colon distention by histomorphometry and scan electronic microscopy (SEM). Sixteen horses were used. In the distended group (DG), nine of them were subjected to distention of the small colon by a surgically implanted intraluminal balloon that was inflated with a pressure of 40mm Hg during 4 hours. In the sham-operated group (SG), the balloon was implanted but not inflated. Full-thickness intestinal samples were collected before and after obstruction and after 1.5 and 12 hours of decom-pression. By SEM, it was observed that the mucosa turned flat and smooth after distention and returned to the wrinkled original appearance after decompression. Twelve hours after decompression the mucosa had a more irregular appearance with points of fragmentation. There was a reduction in mucosa thickness after distention, returning to basal values after decompression. Instead of the fact that there were changes in appearance and thickness, it was concluded that the mucosa could borne up the compression caused by distention returning to the original characteristics without major lesions.